Process for identifying modulators of G-protein-coupled...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C435S007210, C436S501000

Reexamination Certificate

active

11060023

ABSTRACT:
The invention relates to a widely applicable process for identifying chemical compounds, which modulate G-protein-coupled receptors, by means of novel hybrid G-proteins with broad receptor specificity and very high expression and also to chemical compounds which can be identified by such a process.

REFERENCES:
patent: WO97/48820 (1997-12-01), None
patent: WO99/05177 (1999-02-01), None
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Coward et al., Chimeric G Proteins Allow a High-Throughput Signaling Assay of G-coupled Receptors, Analytical Biochemistry, vol. 270, 1999, pp. 242-248.
Erlenbach et al., Single Amino Acid Substitutions and Deletions that Alter the G Protein Coupling Properties of the V2 Vasopessin Receptor Identified in Yeast by Receptor Random Mutagenesis, Journal of Biological Chemistry, vol. 276, 2001, pp. 29382-29392.
Kostenis et al., Functional Characterization of a Series of Mutant G Protein alphaq Subunits Displaying Promiscuous Receptor Coupling Properties, Journal of Biological Chemistry, vol. 273, 1998, pp. 17886-17892.
Kostenis et al., The N-terminal Extension of the Galphaq is Critical for Constraining the Selectivity of Receptor Coupling, Journal of Biological Chemistry, vol. 271, 1997, pp. 19107-19110.
MacDonald, C., Chapter 5: Primary culture and the establishment of cell lines, Basic Cell Culture, J.M. Davis, Ed., IRL Press, 1994, pp. 149-156.
McAteer et al., Chapter 4: Basic cell culture technique and the maintenance of cell lines, Basic Cell Culture, J.M. Davis, Ed., IRL Press 1994, pp. 93-122.
Migeon et al., Regulation of cAMP-mediated Gene Transcription by Wild Type and Mutated G-protein alpha Subunits, Journal of Biological Chemistry vol. 269, No. 46, 1994, pp. 29146-29152.
Wedegaertner et al., Palmitoylation is Required for Signaling Functions and Membrane Attachment of Gqalpha and Gsalpha, J. Biol. Chem, vol. 268, No. 33, 1993, pp. 25001-25008.

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