Process for identifying and enriching cell-specific target...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C435S007800, C435S007200, C435S043000

Reexamination Certificate

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06974675

ABSTRACT:
This invention relates to a process for identifying and enriching cell-specific target structures, in particular for the identification of cell-specific protein combination patterns on the surface of cells and for enriching such cells. The process comprises depositing a heterogeneous cell mixture one or plural surfaces with predefined structures causing cells with corresponding target structures to become bound to such surface(s), removing any non-binding cells of said cell mixture from said surface, identifying the cell-specific target structures responsible for the binding of the cells to said surface(s), selecting and enriching cells with identical cell-specific target structures on said surface(s), and biochemically characterizing the target structures.

REFERENCES:
patent: 6150173 (2000-11-01), Schubert
patent: 197 09 348 (1997-03-01), None
patent: 810 428 (1997-12-01), None
Galati, G. et al., “Quantitative Cytometry of MHC Class I Digestion from Living Cells”, Cytometry, vol. 27, pp. 77-83, (1997).
Zacco, E. et al., “Isolation, Biochemical Characterization and Ultrastructural Analysis of the Limbic System-Associated Membrane Protein (LAMP), a Protein Expressed by Neurons Comprising Functional Neural Circuits”, Journal of Neurosciences, vol. 10(1), pp. 73-90, (1990).
Zhu, Q. et al., “New Cell Surface Marker of the Rat Floor Plate and Notochord”, Developmental Dynamics, vol. 221, pp. 314-326, (1998).
Schubert, W. et al., “Characterization and Distribution of New Cell Surface Marker of Neuronal Precursors”, Developmental Neuroscience, vol. 22, pp. 154-166, (2000).
McGarry, R. et al., “The Characterization and Cellular Distribution of a Family of Antigens Related to Myelin Associated Glycoprotein in the Developing Nervous System”, Journal of Neuroimmunology, vol. 10, pp. 101-114, (1985).
Molecular Cell Biology, J. Darnell et al.,Scinetific American Books, 2nded. 1990, p. 1038.

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