Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2006-09-08
2009-06-30
Goldberg, Jeanine A (Department: 1634)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091100, C435S091200, C435S091210, C536S023100, C536S024310, C536S024330
Reexamination Certificate
active
07553627
ABSTRACT:
There is disclosed an improved high-throughput and quantitative process for determining methylation patterns in genomic DNA samples based on amplifying modified nucleic acid, and detecting methylated nucleic acid based on amplification-dependent displacement of specifically annealed hybridization probes. Specifically, the inventive process provides for treating genomic DNA samples with sodium bisulfite to create methylation-dependent sequence differences, followed by detection with fluorescence-based quantitative PCR techniques. The process is particularly well suited for the rapid analysis of a large number of nucleic acid samples, such as those from collections of tumor tissues.
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Carroll Cindy A.
Danenberg Kathleen D.
Laird Peter W.
Davis , Wright, Tremaine, LLP
Davison, J.D. Barry L.
Goldberg Jeanine A
University of Southern California, USC Stevens Center for Innova
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