Process for growing human epidermis, product thereof

Chemistry: molecular biology and microbiology – Differentiated tissue or organ other than blood – per se – or... – Including perfusion; composition therefor

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4352402, 43524025, 43524031, 435268, 435270, A61K 3536, A61K 3712, C12N 500, C12N 502

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047693177

ABSTRACT:
Procedures are disclosed for eliminating immune competent cells from a population of human epidermal cells. The cells free of immune competent cells are used for growing tissue sheets suitable for transplantation to recipients unrelated to the donor.

REFERENCES:
patent: 4254266 (1981-03-01), Eisinger et al.
patent: 4304866 (1981-12-01), Green et al.
Morhenn et al., "Cultured Epidermal Cells Do Not Synthesize HLA-Dr" J. Invest. Derm., vol. 78, pp. 32-37 (1982).
Klameskog et al., "Ia Antigen Expression on Human Epidermal Langerhans Cells", Nature, vol. 268, pp. 247-250 (1977).
Eisinger et al., "Human Epidermal Cell Cultures . . . " Proc. Nat. Acad. Sci., U.S.A., vol. 76, No. 10, 5340-5344 (1979).
Eisinger et al., "Wound Coverage by a Sheet of Epidermal Cells . . . " Surgery, vol. 88, No. 2, pp. 287-293 (1980).
Hefton et al. "Guinea Pig Epidermal Cell Cultures", Federation Proceedings, vol. 39, pp. 736 (1980).
"Human Epidermal Cells No Longer Stimulate Allogeneic Lymphocytes . . . ", J. Inves. Derm., vol. 76, pp. 308 (1981).

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