Process for growing human epidermal cells in tissue culture

Details Process for growing human epidermal cells in tissue culture Process for growing human epidermal cells in tissue culture

1979-09-13

1981-03-03

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

424 95, 435 1, 435235, C12N 500

Patent

active

042542260

ABSTRACT:
Human epidermal cells are grown in tissue culture by separating the epidermis in human skin from the dermis, dissociating the epidermis into epidermal cells, and growing the epidermal cells in a tissue culture medium having a pH of from about 5.6 to about 5.8.

REFERENCES:
Willmer, Cells & Tissue in Culture vol. 1, pp. 24 and 25 (1965).
Kitano et al., Biochemistry of Cutaneous Epidermal Differentiation, Ed. by Seiji pp. 319-335 (1977).
Eagle, Science vol. 174, pp. 501-503, Oct. 29, 1971.
Eagle et al., J. Cellular Physiology, vol. 82, No. 1, pp. 1-7, Aug. 1973.
Rheinwald et al., Cell, vol. 6, pp. 313-334, (Nov. 1975).

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