Process for fractionating polyethylene glycol (PEG)-protein addu

Details Process for fractionating polyethylene glycol (PEG)-protein addu Process for fractionating polyethylene glycol (PEG)-protein addu

1993-05-12

1994-09-20

Russel, Jeffrey E.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Lymphokines, e.g., interferons, interlukins, etc.

530410, 530423, C07K 308, C07K 312, C07K 1506

Patent

active

053490525

ABSTRACT:
The invention relates to fractionating a mixture of polyethylene glycol (PEG)-protein adducts having different degrees of PEG substitution by partitioning the PEG-protein adducts in a PEG-containing aqueous biphasic system according to the degree of PEG substitution. A new PEG-gm-CSF obtained by the process is useful in pharmaceutical compositions for use in therapeutic or diagnostic methods.

REFERENCES:
patent: 4740304 (1988-04-01), Tjerneld et al.
patent: 4766106 (1988-08-01), Katre et al.
patent: 4904584 (1990-02-01), Shaw
Abuchowski et al., J. Biol. Chem. (1977) 252(11):3582-3586.
Karr et al, "Cell Separation By Immunoaffinity Partitioning With Polyethylene Glycol-Modified Protein A . . . ". J. Chromatography 442 (1988), pp. 219-227.
Chemical Abstracts, vol. 109, No. 9, Aug. 29, 1988, (Columbus, Ohio, US), L. Karr et al., "Cell Separation by Immunoaffinity Partitioning With Polyethylene Glycol-Modified Protein A in Aqueous Polymer Two-Phase Systems". Abstract No. 109:69845r.
Chemical Abstracts, vol. 90, No. 21, May 21, 1979, (Columbus, Ohio, US), A. Chaabouni et al., "Affinity Partition of Proteins in Aqueous Two-Phase Systems Containing Polyoxyethylene Glycol-Bound Ligand and Charged Dextrans", see p. 232, Abstract 164184x, & J Chromatogr. (1979) 171:135-143.

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