Process for filling tubes for cryogenically preserving biologica

Details Process for filling tubes for cryogenically preserving biologica Process for filling tubes for cryogenically preserving biologica

1992-12-23

1994-02-01

Housel, James C.

Chemistry: molecular biology and microbiology

Differentiated tissue or organ other than blood, per se, or...

Including perfusion; composition therefor

422 40, 422 1, 62 60, A01N 102

Patent

active

052831701

ABSTRACT:
The tube, known as a straw, according to the invention, is conventional in its form, and is constituted by a rectilinear length of transparent tubular envelope. In the vicinity of a first end, the straw contains a sliding stopper with a quantity of powder gellable by hydration between two porous pads. The tubular envelope is extruded from an ionomer resin marketed under the brand name of Surlyn 8921. Ionomer resins have, above and below a transition zone extending from 45.degree. C. to 60.degree. C. approximately, states that are respectively thermofusible and crosslinked. Thus, the straw can be closed by fusion of the ends clamped by heated jaws, while presenting suitable properties of rigidity at ambient temperature. Furthermore, the ionomer resin used does not have an embrittlement temperature, so that it can be manipulated at cryogenic temperatures (77 K.) without the need for taking any particular precautions. The filling and sealing of the straws can be automated, to ensure the safety of the operators.

REFERENCES:
patent: 3404134 (1968-10-01), Rees
patent: 3579303 (1968-03-01), Pickering
patent: 5190880 (1993-03-01), Cassou et al.

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