Process for evaluating the global capacity of urine to form chal

Details Process for evaluating the global capacity of urine to form chal Process for evaluating the global capacity of urine to form chal

1998-09-08

2000-02-22

Snay, Jeffrey

Chemistry: analytical and immunological testing

Metal or metal containing

Present in biological fluids

436 79, 436164, 436177, 422 61, G01N 2129

Patent

active

060279397

DESCRIPTION:

BRIEF SUMMARY
TECHNICAL FIELD OF THE INVENTION

The present invention is inserted in the field of study of the risk of calcic renal lithiasis, specifically on the development of a kit type system that allows the evaluation of the capacity of forming calcic crystals in a urine sample of any individual.


PRIOR ART OF THE INVENTION

The formation of renal calculus, as it is known, is due in practically all cases to the unfortunate combination of several factors. These factors may be classified in two large groups: I) factors inherent to the composition of urine. II) factors related to the morpho-anatomy of the kidney.
Urine is a metastable medium where there are normally different substances that may crystallize forming calculus (substances that are found in a state of supersaturation). Whether or not these substances crystallize depends on the degree of supersaturation, the presence of promoting substances (heterogeneous nucleants) and the presence of crystallization inhibitors.
The presence of cavities with a low urodynamic efficacy and that, therefore, keep the urine retained for long periods of time, and the alterations of the epithelium that covers the renal papilla (reduced or eliminated layer of glycosaminoglykanes, necrosis, . . . ) are factors linked to the renal structure that favor the formation of renal calculus.
Normally, the existence of factors belonging to both groups is necessary for the formation of renal calculus. The test that is presented precisely makes it possible to evaluate in a very simple manner the capacity that specific urine has to crystallize calcic salts, in such a way that the urine of a healthy individual would not give rise to growth of calcic salt crystals, while urine tending to form renal calculus (very supersaturated and/or with a significant inhibition deficit and/or abundant heterogeneous nucleants) would produce calcic salt crystals.
As bibliographic references closely related to the object of the present invention, the following may be cited: J. M. Baumann. How reliable are the measurements of crystallization conditions in urine? Urol. Res. 16, 133-135 (1988); J. M. Baumann. How to measure crystallization conditions in urine: A comparison of 7 methods. Report from a workshop held on Nov. 28, 1987 in Basle. Urol. Res 16, 137-142 (1988); F. Grases, O. Sohnel. Mechanism of oxalocalcic renal calculi generation, Int. Urol. Nephrol. 25, 209-214 (1993); F. Grases, A. Costa-Bauza, J. G. March, O. Sohnel, Artificial simulation of renal stone formation. Influence of some urinary components. Nephron 65, 77-81 (1993); F. Grases, A. Costa-Bauza, J. G. March. Artificial simulation of the early stages of renal stone formation. Brit. J. Urol. 74, 298-301 (1994).
ES-A-2088743 discloses a method for the determination of the inhibitive capacity of crystallization in human urine samples and the corresponding kit therefor.
The cited method essentially comprises the following operations: or flat solid surface that initiates crystallization, at 37.degree. C. for about 5-6 hours; (b) removing the substrate containing the crystals and dissolving them in an acid medium; (c) evaluating the amount of calcium contained in said crystals.
The kit used to carry out this method comprises (1) a small vessel containing a substrate (2); (3) a container of the urine to be analyzed; (4) a container where the other container (1) is inserted; (5) a test tube type container, in which the dissolving of the crystals deposited in substrate (2) takes place.
Grases, F. et al (1995) International Urology and Nephrology 27: 653-661 discloses a similar process as that of ES-A-2088743.
Fernadndez-Dapica, M. P. et al. (1994) Arthritis & Rheumatism 370: s143 discloses a method for analyzing the calcium phosphate contents of synovial liquids using ARENAZO III colorimetrical assay. This method is inadequate for providing a simplified process for evaluating the capacity of urine as that of ES-A-2088743 inasmuch the method of Fernandez-Dapica implies digesting the samples of synovial fluid with NaOH at high temperatures (about 100.deg

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Grases et al.,1995, Intl Urol Nephrol, 27:653-61.

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