Process for evaluating chemical and biological assays

Details Process for evaluating chemical and biological assays Process for evaluating chemical and biological assays

2006-08-08

2006-08-08

Brusca, John S. (Department: 1631)

Data processing: measuring, calibrating, or testing

Measurement system in a specific environment

Biological or biochemical

C435S006120

Reexamination Certificate

active

07089120

ABSTRACT:
An analytical process is disclosed, for discriminating data acquired from samples with overlapping distributions, and for improving and assessing the statistical validity of hybridization signal in arrays of assays. The process includes method of convolving data into two or more discrete probability density functions representing signal and nonsignal, discrete fluors, or other convolved independent variables. The system uses the probability density functions to assign hybridization signals, objectively, to one of the modeled distributions. Subsequent processes assess variability inherent to the arrays, and use this assessed variation to establish reliability scores and confidence limits for complete hybridization arrays, and for discrete hybridization assays within arrays.

REFERENCES:
Chen et al. Ratio-Based Decisions and the Quantitative Analysis of cDNA Microarray Images. Journal of Biomedical Optics Vo 2 pp. 364-374 (1997).

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