Process for esterification of .alpha.-L-aspartyl-L-phenylalanine

Details Process for esterification of .alpha.-L-aspartyl-L-phenylalanine Process for esterification of .alpha.-L-aspartyl-L-phenylalanine

1980-09-02

1981-10-06

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Using tissue cell culture to make a protein or polypeptide

435128, 435135, C12P 2102

Patent

active

042936489

ABSTRACT:
A process for selectively esterifying .alpha.-L-aspartyl-L-phenylalanine to the corresponding alkyl esters using a proteolytic enzyme with specific esterase activity is described. The process is carried out in an aqueous-alcohol medium in which the alcohol concentration is sufficient to reverse the esterase activity. The .alpha.-L-aspartyl-L-phenylalanine alkyl esters so produced are useful as artificial sweeteners.

REFERENCES:
Ingalls et al., Biotechnology & Bioengineering, vol. 17, pp. 1627-1637 (1975).
Klibanov et al. Biotechnology & Bioengineering, vol. 19, pp. 1351-1361 (1977).

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