Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;... – Enzyme or microbial cell is immobilized on or in an organic...
Patent
1993-02-10
1994-08-30
Naff, David M.
Chemistry: molecular biology and microbiology
Carrier-bound or immobilized enzyme or microbial cell;...
Enzyme or microbial cell is immobilized on or in an organic...
435180, 436532, 530413, 530816, C12N 1106, C12N 1108, G01N 33549, C07K 1700
Patent
active
053427726
ABSTRACT:
The fluorocarbon surface of a solid or liquid support is activated with a highly fluorinated isocyanate-modified ligand or with a reactive poly(fluoroalkyl) sugar reagent containing a polyhydroxy sugar to which are attached a plurality of fluoroalkyl anchor groups, a reactive group and optionally a spacer. The activated support has application in separation of biomolecules, immobilization of biomolecules, heterogeneous diagnostic assays, and biosensors. An enzyme or other biomolecule is immobilized by contacting the activated support surface with the enzyme in the presence of a surfactant. The surfactant is preferably a neutral surfactant such as a fluoroalkyl-polyoxyethylene.
REFERENCES:
patent: 3843443 (1974-10-01), Fishman
patent: 4317879 (1982-03-01), Busby et al.
patent: 4619897 (1986-10-01), Hato et al.
patent: 4619904 (1986-10-01), Giaever et al.
patent: 4885250 (1989-12-01), Eveleigh et al.
patent: 4954444 (1990-09-01), Eveleigh et al.
patent: 5079155 (1992-01-01), Cox et al.
DeMiguel et al., Chromatographia, 24:849-853 (1978).
Danielson, et al, Biotechnol. Bioeng. 23, 1913-1917 (1981).
Siergeiej, Dissertation Abstracts Int. B, vol. 44, 153 (1983).
Chem. Abstracts, vol. 108, 1975579, No. 22, May 30, 1988, p. 741
Kennedy, et al., Solid Phase Biochemistry, Analytical and Synthetic Aspects, Scouten, Ed., (1983) p. 253.
Arenzen Rene
Boivin Patrick
Kobos Robert K.
Scouten William H.
Smart Bruce E.
Baylor University
E. I. Du Pont de Nemours and Company
Naff David M.
LandOfFree
Process for enzyme immobilization on a fluorocarbon surface does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Process for enzyme immobilization on a fluorocarbon surface, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for enzyme immobilization on a fluorocarbon surface will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-29513