Process for enzymatically converting glycolonitrile to...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound

Reexamination Certificate

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C435S183000, C435S227000, C530S350000

Reexamination Certificate

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07927847

ABSTRACT:
A process is provided to improve the specific activity of an enzyme catalyst having nitrilase activity when converting glycolonitrile to glycolic acid under aqueous reaction conditions. Inclusion of an effective amount of at least one amine protectant improves the specific activity and catalytic productivity of the enzyme catalyst.

REFERENCES:
patent: 2175805 (1939-10-01), Jacobson
patent: 2890238 (1959-06-01), Sexton
patent: 3940316 (1976-02-01), Commeyras et al.
patent: 5187301 (1993-02-01), Cullen et al.
patent: 5223416 (1993-06-01), Endo et al.
patent: 5234826 (1993-08-01), Yamagami et al.
patent: 5296373 (1994-03-01), Endo et al.
patent: 5326702 (1994-07-01), Endo et al.
patent: 5508181 (1996-04-01), Hashimoto
patent: 5605793 (1997-02-01), Stemmer
patent: 5756306 (1998-05-01), Yamaguchi et al.
patent: 5811238 (1998-09-01), Stemmer et al.
patent: 5814508 (1998-09-01), DiCosimo et al.
patent: 5830721 (1998-11-01), Stemmer et al.
patent: 5837458 (1998-11-01), Minshull et al.
patent: 6037155 (2000-03-01), Kobayashi et al.
patent: 6291708 (2001-09-01), Cockrem
patent: 6383786 (2002-05-01), Chauhan et al.
patent: 6416980 (2002-07-01), Chauhan et al.
patent: 6870038 (2005-03-01), Chauhan et al.
patent: 7148051 (2006-12-01), Payne et al.
patent: 7198927 (2007-04-01), DiCosimo et al.
patent: 7473545 (2009-01-01), DiCosimo et al.
patent: 7638617 (2009-12-01), DiCosimo et al.
patent: 2004/0138409 (2004-07-01), Hayashi et al.
patent: 2004/0210087 (2004-10-01), Meng et al.
patent: 2006/0160196 (2006-07-01), Foo
patent: 2006/0247467 (2006-11-01), DiCosimo
patent: 0486289 (1992-05-01), None
patent: 546049 (1995-07-01), None
patent: 0610048 (1999-09-01), None
patent: 0610049 (1999-11-01), None
patent: 4099495 (1992-03-01), None
patent: 4099496 (1992-03-01), None
patent: 4099497 (1992-03-01), None
patent: 4218386 (1992-08-01), None
patent: 5095795 (1993-04-01), None
patent: 5192189 (1993-08-01), None
patent: 6/0156086 (1994-06-01), None
patent: 6237789 (1994-08-01), None
patent: 6284899 (1994-10-01), None
patent: 7/213295 (1995-08-01), None
patent: 09028390 (1997-02-01), None
patent: WO93/24631 (1993-12-01), None
patent: WO01/75077 (2001-10-01), None
patent: WO 02/068658 (2002-09-01), None
patent: WO2005106005 (2005-11-01), None
patent: WO2006/069114 (2006-06-01), None
patent: WO2006068110 (2006-06-01), None
Metz et al., “Identification of Formaldehyde-induced Modifications in Proteins,” J. Biol. Chem., 279(8), 6235-6243, 2004.
Asano et al, “Aliphatic Nitrile Hydratase from Arthrobacter sp. J-1 Purification and Characterization,” Agricultural Biological Chemistry, 46(5), 1165-1174, 1982.
Mowry, David, “The Preparation of Nitrites,” Chemical Reviews, 42, 189-283, 1948.
Tourneix et al., Antonie van Leeuwenhoek, 52, 173-182, 1986.
Chauhan et al., Purification, cloning, sequencing and over-expression inEscherichia coliof a regioselective aliphatic . . . , Appl. Microbiol Biotechnol., 61, 118-122, 2003.
Athel Cornish-Bowden,“Nomenclature for incompletely specified bases in nucleic acid Sequences: recommendations 1984,”Nucleic Acids Res. 13, 3021-3030, 1985.
“Nomenclature and Symbolism for Amino Acids and Peptides,” Biochemical Journal, 219(2), 345-373, 1984.
Bickerstaff, G.F., Immobilization of Enzymes and Cells, Methods in Biotechnology, Ch. 4, Ch. 5, Ch. 17, Ch. 32, pp. 27-40, 41-51, 133-141, 289-298,1997. (respectively).
Wong, S.S., “Chemistry of Protein Conjugation and Crosslinking,” CRC Press, Inc., Ch. 12, pp. 295-317, 1991.
Outchkourov et al., “Optimization of the Expression of Equistatin inPichia pastoris,” Protein Expr.Purif, 24(1), 18-24, 2002.
Feng et al., High-Level Expression and Mutagenesis of Recombinant Human Phophatidylcholine Transder Protein Using a Synthetic Gene . . . , Biochemistry, 39(50), 15399-15409, 2000.
Pace et al., “The nitrilase superfamily: classification, structure and function,” Genome Biol., 2(1), reviews 0001.1-0001.9, 2001.
Cowan et al., “Biochemistry and Biotechnology of mesophilic and thermophilic nitrile metabolizing enzymes,” Extremophiles, 2, 207-216, 1998.
Deshpande, M. V., Ethanol Production from Cellulose by Couple Saccharification/Fermentation . . . , Appl. Biochem. Biotechnol., 36(3), 227-234, 1992.
Chica et al., Semi-rational approaches to engineering enzyme activity . . . , Curr. Opin. Biotechnol., Aug. 16(4):378-84, 2005.
Sen et al, Developments in Directed Evolution for Improving Enzyme Functions, Appl. Biochem. Biotechnol., Dec.:143(3):212:23, 2007.
Desantis, et al., “Creation of a productive, highly enantioselective nitrilase through gene site saturation mutagenesis”, J. Am. Chem. Soc., vol. 125, pp. 11476-11477 (2003).
Fromant, M., et al., “Direct random mutagenesis of gene-sized DNA fragments using polymerase chain reaction”, Analytical Biochem., vol. 224, pp. 347-353 (1995).
Guthrie, J.P., et al., “Effect of the ACYL substituent on the equilibrium constant for hydration of esters”, Canadian J. Chem., vol. 58, pp. 1281-1294 (1980).
Inci, I., “Distribution of glycolic acid between water and different organic solutions”, Chem. Biochem. Eng. Q., vol. 16, pp. 81-85 (2002).
Inci, I., et al., “Extraction of glycolic acid from aqueous solutions by trioctyl methylammonium chloride and organic . . . ”, J. Chem. Eng. Data, vol. 50, pp. 536-540 (2005).
Lin-Goerke, “PCR-based random mutagenesis using manganese and reduced DNTP concentration”, Biotechniques, vol. 23, pp. 409-412 (1997).
Melnikov, A., et al., “Random mutagenesis by recombinational capture of PCR products inBacillus subtilisand . . . ”, Nucleic Acids Res., vol. 27, pp. 1056-1062 (1999).
O'Reilly, C., et al., “The nitrilase family of CN hydrolyzing enzymes-a comparative study”, J. Appl. Microbiol., vol. 95, pp. 1161-1174 (2003).
Tamada, J.A., et al., “Extraction of carboxylic acids with amine extractants. 1. Equilibria and law of mass action . . . ”, Ind. Eng. Chem. Res., vol. 29, pp. 1319-1326 (1990).
Tamada, J.A., et al., “Extraction of carboxylic acids with amine extractants. 2. Chemical interactions and . . . ”, Ind. Eng. Chem. Res., vol. 29, pp. 1327-1333 (1990).
Wasewar, K.L., et al., “Reactive extraction of lactic acid using alamine 336 in MIBK: equilibria and kinetics”, J. Biotechnol., vol. 97, pp. 59-68 (2002).
Yanisch-Perron, C., et al., “Improved M13 phage cloning vectors and host strains: nucleotide sequences . . . ”, NCBI Gen. ID No. 20141090, Accession No. L09137, May 22, 2002.
Notice of Allowance in U.S. Appl. No. 12/210,618, now U.S. Pat. No. 7,638,617, mailed Aug. 27, 2009.
Requirement for Restriction/Election in U.S. Appl. No. 11/712,336, now U.S. Pat. No. 7,473,545, mailed Mar. 28, 2008.
Notice of Allowance in U.S. Appl. No. 11/712,336, now U.S. Pat. No. 7,473,545, mailed Aug. 29, 2008.
Requirement for Restriction/Election in U.S. Appl. No. 11/313,541, now U.S. Pat . No. 7,198,927, mailed Aug. 14, 2006.
Notice of Allowance in U.S. Appl. No. 11/313,541, now U.S. Pat . No. 7,198,927, mailed Nov. 30, 2006.

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