Process for enzymatic replacement of the B-30 amino acid in insu

Details Process for enzymatic replacement of the B-30 amino acid in insu Process for enzymatic replacement of the B-30 amino acid in insu

1983-01-18

1986-04-01

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Using a micro-organism to make a protein or polypeptide

435 69, C12P 2104, C12P 2106

Patent

active

045798206

ABSTRACT:
An insulin such as porcine insulin is reacted enzymatically with an L-amino acid, amide, or ester in the presence of L-specific serine carboxypeptidase modified by reaction with divalent metal ions in aqueous solution or dispersion containing F.sup.-, Cl.sup.-, Br.sup.-, I.sup.-, CN.sup.-, or SCN.sup.-.

REFERENCES:
patent: 3276961 (1966-10-01), Bodanszky
patent: 4339534 (1982-07-01), Johansen et al.
Breddam, K. et al., Carlsberg Res. Commun., vol. 46(6), p. 361-372, (1981), Chem. Abst. 97:110372h.
Hayashi, R., Bai, Y. and Hata, T.: Further Confirmation of Carboxypeptidase Y as a Metal-Free Enzyme having a Reactive Serine Residue, J. Biochem., 77, 1313-1318, (1975).
Bai, Y. and Hayashi, R.: Properties of the Single Sulfhydryl Group of Carboxypeptidase Y, The Journal of Biological Chemistry, vol. 254, No. 17, Sep. 10, pp. 8473-8479, 1979.
Widmer, F., Johansen, J. T.: Enzymatic Peptide Synthesis Carboxypeptidase Y Catalyzed Formation of Peptide Bonds, Carlsberg Res. Commun., vol. 44, Apr. 23, 1979, 37-46.
Johansen, J. T., Breddam, K., Ottesen, M.: Isolation of Carboxypeptidase Y by Affinity Chromatography, Carlsberg Res. Commun., vol. 41, No. 1, 1976, 1-13.
Breddam, K., Widmer, F., Johansen, J. T.: Carboxypeptidase Y Catalyzed Transpeptidations and Enzymatic Peptide Synthesis, Carlsberg Res. Comm., vol. 45, Nov. 5, 1980, pp. 237-247.
Kubota et al.: Carboxypeptidase C.sub.N, J. Biochem., vol. 74, No. 4, (1973), pp. 757-770.
Widmer, F., Breddam, K., Johansen, J. T.: Influence of the Structure of Amino Components on Carboxypeptidase Y Catalyzed Amide Bond Formation, Carlsberg Res. Comm., vol. 46, Apr. 29, 1981, pp. 97-106.
Lee, H. M., Riordan, J. F.: Does Carboxypeptidase Y have Intrinsic Endopeptidase Activity? Biochemical and Biophysical Research Comm., vol. 85, No. 3, 1978, 1135-1142.

Affiliated with

Also associated with

Rating

Process for enzymatic replacement of the B-30 amino acid in insu does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Process for enzymatic replacement of the B-30 amino acid in insu, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for enzymatic replacement of the B-30 amino acid in insu will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1084448