Process for eluting egg white lysozyme

Details Process for eluting egg white lysozyme Process for eluting egg white lysozyme

1982-11-23

1985-05-21

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435815, C12N 936

Patent

active

045186952

ABSTRACT:
In the process of eluting egg white lysozyme by contacting a weakly acidic cation exchange resin having egg white lysozyme adsorbed thereon with an eluting agent comprising a salt solution, an alkali agent is added to the eluate containing the weakly acidic cation exchange resin having egg white lysozyme adsorbed thereon whereby the egg white lysozyme can be eluted more effectively. From the eluate thus obtained, lysozyme can be collected in a high yield by an ordinary collecting method such as salting out.

REFERENCES:
patent: 2579455 (1951-12-01), Alderton et al.
patent: 3515643 (1970-06-01), Ghielmetti et al.
patent: 4104125 (1978-08-01), Takechi et al.
Hackh's Chemical Dictionary, 4th Edition, 1969, p. 239.

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