Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2005-06-28
2005-06-28
Le, Long V. (Department: 1641)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C436S017000, C436S018000, C436S056000, C436S063000, C436S164000, C436S172000, C436S175000, C436S176000, C436S177000, C435S002000, C435S006120, C435S007210, C435S007240, C435S007250
Reexamination Certificate
active
06911313
ABSTRACT:
A method for discriminating and counting erythroblasts comprises the steps of: (i) staining leukocytes in a hematologic sample by adding a fluorescent labeled antibody capable of binding specifically with leukocytes to the hematologic sample; (ii) raising the permeability only of cell membranes of erythroblasts in the hematologic sample to a nucleotide fluorescent dye which does not permeate a cell membrane usually, the nucleotide fluorescent dye having a fluorescent spectrum capable of being distinguished from that of a fluorescent labeling compound of the fluorescent labeled antibody in step (i); (iii) staining nuclei of the erythroblasts in the hematologic sample with the nucleotide fluorescent dye; (iv) subjecting the hematologic sample to flowcytometry to detect at least two fluorescent signals from each cell; and (v) discriminating and counting the erythroblasts from difference in intensity between the at least two fluorescent signals.
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Lehninger, Albert L., Principles of Biochemistry, (1982), p. 55.
Hamaguchi Yukio
Houwen Berend
Sakata Takashi
Tsuji Tomohiro
Wang Fu-sheng
Bryan Cave LLP
Gabel Gailene R.
Le Long V.
Sysmex Corporation
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