Process for directly determining apurinic and apyrimidinic sites

Details Process for directly determining apurinic and apyrimidinic sites Process for directly determining apurinic and apyrimidinic sites

1984-03-22

1989-09-05

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 18, 436 94, C12Q 168, C12Q 134

Patent

active

048638471

ABSTRACT:
A simple and fast process is described which serves to directly determine apurinic and apyrimidinic sites (AP sites) in DNA using [.sup.14 C]methoxyamine which, after loss of a purine or pyrimidine base, respectively, reacts with the resultant aldehyde groups of the deoxyribose groups.
The incorporation of the [.sup.14 C]methoxyamine in DNA is proportional to the number of AP sites. Since methoxyamine does not cause the DNA to degrade, the unreacted AP sites can be measured in order to determine the radioactivity of the acid-insoluble fraction.
The process lends itself to the analysis of DNA-damages such as those which are physically or chemically produced. Moreover it is suitable to demonstrate and determine the activity of DNA glycosylases, in particular of uracil-DNA glycosylases.

REFERENCES:
Coombs et al, Biochim. Biophys. Acta, 174: 161-173 (1969).
Talpaert-Borle et al, Biochim. Biophys. Acta, 740: 410-416 (Sep. 9, 1983).
Talpaert-Borle et al, J. Biol. Chem., 257(3): 1208-1214 (1982).
Talpaert-Borle et al, J. Biol. Chem., 254(14): 6387-6391 (1979).
J. of Virology, 40(1): 204-210 (1981), H. Warner et al., "Evidence that the UV Endonuclease Activity Induced by Bacteriophage T4 Contains Both Pyrimidine Dimer DNA, Glycosylase and Apyrimidinic/Apurinic Endonuclease Activities in the Enzyme Molecule".
Proc. Natl. Acad. Sci. USA., 78(5): 2742-2746 (1981), Y. Nakabeppu et al., "Physical Association of Pyrimidine Dimer DNA Glycosylase and Apurinic/Apyrimidinic DNA".

Affiliated with

Also associated with

Rating

Process for directly determining apurinic and apyrimidinic sites does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Process for directly determining apurinic and apyrimidinic sites, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for directly determining apurinic and apyrimidinic sites will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-243121