Process for differentiating the origin of particles in urine

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 29, 436519, 436520, 436804, 436811, G01N 3350, C12Q 102

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045754861

ABSTRACT:
Urinary particulates in a human subject which have been coated with THP is determined to be from the region of the nephron which is above the Henle loop in the nephron where THP is produced. The identification of those particles in the urine sample which had been coated with THP is performed by subjecting the sample to THP antibodies to form THP antibody-antigen complexes. These complexes can be identified by a number of ways, such as visual, fluorescent, etc. By determining the percentage of the total particulates which have the coating of THP, a diagnostic tool for determining renal disorders is disclosed.

REFERENCES:
Hanson et al., Lancet, 1: 226-228 (1976).
Rutecki et al., New Eng. J. Med., 284(19): 1049-1052 (1971).
Bartalos et al., Chemical Abstracts, 94:79565k, (1981).
Marier et al., Chemical Abstracts, 90:119095h, 432 (1979).
"Direct Visualization of a Mucoprotein Component of Urine" by Keith R. Porter and Igor Tamm, The Journal of Biological Chemistry, vol. 212, No. 1, Jan. 1955; pp. 135-139.
"Ultracentrifugation Studies of a Urinary Mucoprotein which Reacts with Various Viruses" by Igor Tamm, J. C. Bugher and F. L. Horsfall, Jr. The Journal of Biological Chemistry, vol. 212, No. 1, Jan. 1955; pp. 125-133.
"Tamm-Horsfall Urinary Glycoprotein" by A. P. Fletcher, A. Neuberger & Wendy A. Ratcliffe, Journal of Biochemistry, vol. 120, 1970; pp. 425-432.
"Tamm-Horsfall Urinary Glycoprotein, The Chemical Composition" by A. P. Fletcher, A. Neuberger & Wendy A. Ratcliffe; Journal of Biochemistry, vol. 120, 1970; pp. 417-424.
"Characterization and Spearation of an Inhibitor of Viral Hemagglutination Present in Urine" by Igor Tamm and Frank L. Horsfall, Jr. PSEBM, vol. 74, 1950; pp. 108-114.

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