Process for determining the quantity of a DNA fragment of intere

Details Process for determining the quantity of a DNA fragment of intere Process for determining the quantity of a DNA fragment of intere

1994-06-21

1998-05-05

Houtteman, Scott W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435912, C12Q 168

Patent

active

057472467

ABSTRACT:
The present invention relates to a process for determining the quantity of a DNA fragment of interest in a sample wherein:

REFERENCES:
patent: 5082935 (1992-01-01), Cruickshank
Kemp et al. Colorimetric Detection of Specific DNA Segments Amplified by ymerase Chain Reactions. Proc. Natl. Acad. Sci. USA (Apr. 1989) 86:2423-2427.
Konat, G. et al. Generation of Radioactive and Nonradioactive ssDNA Hybridization Probes by Polymerase Chain Reaction. Technique (1991) 3:64-68.
Norval, M., et al. Advances in the Use of Nucleic Acid Probes in Diagnosis of Viral Diseases of Man Brief Review. Arch. V.201 (1987) 97:151-165. (Abstract).
Nucleic Acids Research, vol. 17, No. 22, 1989, pp. 9437-9446, M. Becker-Andre, et al., "Absolute mRNA Quantification Using the Polymerase Chain Reaction (PCR). A Novel Approach by a PCR Aided Transcript Titration Assay (PATTY)".
Proceedings of the National Academy of Sciences of USA, vol. 87, Apr. 1990, pp. 2725-2729, G. Gilliland, et al., "Analysis of Cytokine mRNA and DNA: Detection and Quantitation by Competitive Polymerase Chain Reaction".

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