Process for determining superoxide dismutase activity

Details Process for determining superoxide dismutase activity Process for determining superoxide dismutase activity

1986-02-28

1989-01-31

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 4, 435 28, 435189, 435192, C12Q 126, C12Q 128, C12N 902, C12N 908

Patent

active

048015385

ABSTRACT:
Superoxide dismutase activity is determined by measuring hydrogen peroxide produced by using superoxide anion as a substrate and acting superoxide dismutase thereon in the presence of an electron carrier. When maleimide or a derivative thereof, and if necessary, a carbonyl compound, and if further necessary bromine ions and/or citric acid or a salt thereof are added to the electron carrier, the sensitivity and the linearity of calibration curves are further improved.

REFERENCES:
patent: 4384042 (1983-05-01), Miike et al.
Chemical Abstracts, vol. 102, No. 1, Jan. 7, 1985, pp. 415, 416, Abstract No. 4350M.

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