Process for determining superoxide dismutase activity

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase

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435 4, 435 28, 435189, 435192, C12Q 126, C12Q 128, C12N 902, C12N 908

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active

048015385

ABSTRACT:
Superoxide dismutase activity is determined by measuring hydrogen peroxide produced by using superoxide anion as a substrate and acting superoxide dismutase thereon in the presence of an electron carrier. When maleimide or a derivative thereof, and if necessary, a carbonyl compound, and if further necessary bromine ions and/or citric acid or a salt thereof are added to the electron carrier, the sensitivity and the linearity of calibration curves are further improved.

REFERENCES:
patent: 4384042 (1983-05-01), Miike et al.
Chemical Abstracts, vol. 102, No. 1, Jan. 7, 1985, pp. 415, 416, Abstract No. 4350M.

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