Process for determining nuclease activity

Details Process for determining nuclease activity Process for determining nuclease activity

1993-11-05

1996-09-10

Paden, Carolyn

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 4, 435 74, 436172, 436800, 935 76, 935 77, C12Q 100, C12Q 168, G01N 2176, C12N 1500

Patent

active

055545025

ABSTRACT:
The present invention relates to analyzing a test sample for the presence or absence of nuclease activity, and for quantifying the amount of nuclease activity. In one aspect of the invention, a kit is provided which allows the rapid and efficient quantification of nuclease activity in a sample having an unknown nuclease activity. In another aspect of the invention, a method for determining the presence or absence of nuclease activity, and for quantifying nuclease activity is disclosed.

REFERENCES:
Rye et al. Analytical biochemistry 208 pp. 144-150 (1993).
Kurnick, Methods of Biochemical Analysis vol. IX, (1962) Intersciences Publishers John Wiley & Sons.
Analytical Biochemistry, vol. 208, issued Jan. 1993, Rye et al., "Fluorometric Assay Using Dimeric Dyes for Double- and Single-Stranded DNA and RNA with Picogram Sensitivity", pp. 144-150.

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