Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-03-14
1997-07-01
Jones, W. Gary
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 5, 435 912, 435174, 435183, 536 243, 536 2432, 536 2433, C12Q 168, C12Q 170, C12P 1934, C07H 2104
Patent
active
056437307
ABSTRACT:
This invention relates to a process for detecting the presence and measuring the quantity of specific mRNA sequences present in in vivo cells or cells maintained in vitro. The process of this invention is applicable to the screening of procaryotic and eucaryotic organisms including the screening of human beings for the presence of disease states. The process of this invention is also applicable to the in vitro screening of the effect or effects of chemical compounds upon one or several gene products as exhibited by the presence and amount of mRNA resulting from transcription of said gene or genes. The process of this invention is particularly suited for screening of a large number of compounds for the effect or effects of compounds upon gene products. This invention also relates to compounds capable of affecting the presence of specific mRNA sequences in cells. The process of this invention also is applicable to the identification of novel gene constructs in viruses, microorganisms, plants and animals. This invention also relates to a novel process for the isolation of RNA and DNA from cells.
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Banker Michael J.
Davidson Ralph E.
Pereira Dennis A.
Benson Gregg C.
Jones W. Gary
Pfizer Inc.
Rees Dianne
Richardson Peter C.
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