Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1988-05-12
1991-03-05
Teskin, Robin L.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435 29, 435 6, 4351723, 935 76, 935 78, 935 79, 935 84, C12N 1500, C12Q 102, C12Q 168
Patent
active
049977571
ABSTRACT:
There is provided a process for screening an agent in order to determine whether such agent increases the frequency of genome rearrangement in living matter.
In the first step of this process, there is provided a viable species of Saccharomyces cerevisiae yeast which comprises repeated genetic elements in its haploid genome. These repeated genetic elements are selected from the group consisting of functional and non-functional genetic elements; and these elements are sufficiently homologous so that, under ambient conditions, they recombine with each other and give rise to an indentifiable genome rearrangement which is a deletion.
In the second step of this process, the viable species of yeast is exposed to the agent to be tested. Thereafter, it is plated onto a growth medium which, after the exposed yeast species grows upon it, facilitates the identification of those yeast which have undergone said genome rearrangement.
In the last step of the process, the extent to which the exposed species of yeast has undergone genome rearrangement is determined.
Also disclosed is a the viable yeast strain used in said process, the plasmid used to construct said strain, and a process for constructing said strain.
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GeneBioMed, Inc.
Greenwald Howard J.
Teskin Robin L.
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