Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1990-03-14
1992-06-30
Wax, Robert A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 91, 436 94, 436501, 935 77, 935 78, C12Q 168, G01N 3348, G01N 33566, C12P 1934
Patent
active
051262398
ABSTRACT:
A process is provided for detecting polymorphisms on the basis of nucleotide differences in random segments of the nucleic acid by performing a primer extension reaction on the nucleic acids and comparing the extension reaction products. The random nucleic acid segment can be amplified by first dissociating the extension product from the template and contacting the dissociated extension product with a primer under conditions such that an amplification extension product is synthesized using the dissociated extension product as a template. Differences in the extension product are useful as markers in constructing genetic maps and as markers to distinguish or identify individuals.
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Livak Kenneth J.
Rafalski Jan A.
Tingey Scott V.
Williams John G.
E. I. Du Pont de Nemours and Company
Wax Robert A.
Zitomer Stephanie W.
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