Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1983-06-17
1985-12-10
Rosen, Sam
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
C12Q 132
Patent
active
045580073
ABSTRACT:
An improved assay procedure for quantitatively detecting LDH.sub.k isozyme in human serum is provided which utilizes previously known electrophoretic gel separation procedures combined with an improved staining method wherein the staining solution has a constant temperature of about 37.degree. C. and a pH of about 8.0, and is contacted with the gel matrix for at least 3 hours in the absence of oxygen and light. This improved assay procedure may be used as an adjunct to other procedures for diagnosing the presence of primary cancer, as a post-operative indicator of metastatic cancer and in monitoring the success of cancer therapy.
REFERENCES:
patent: 4258131 (1981-03-01), Takagahara et al.
Anderson et al., LDH.sub.k, a Uniquely Regulated Cryptic Lactate Dehydrogenase Associated with Transformation by the Kirsten Sarcoma Virus, J. of Bio. Chem., vol. 256, No. 20, pp. 10583-10591 (Oct., 1981).
Anderson et al., LDH.sub.k, An Unusual Oxygen-Sensitive Lactate Dehydrogenase Expressed in Human Cancer, Proc. Natl. Acad., Sci. USA, vol. 78, No. 5, pp. 3209-3213 (May, 1981).
Dietz et al., Separation and Quantitation of Lactic Dehydrogenase Isoenzymes by Disc Electrophoresis, Analytical Biochemistry 20, 246-257 (1967).
Anderson Garth R.
Manly Kenneth F.
Mittelman Arnold
Health Research, Inc. (Roswell Park Divison)
Rosen Sam
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