Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1988-11-14
1990-06-12
Nucker, Christine M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 29, 435 34, 436 63, 436 64, 436813, G01N 3348
Patent
active
049332740
ABSTRACT:
A process for determining genetic susceptibility to cancer is disclosed in which the frequency of chromatid breaks and gaps is calculated in metaphase skin fibroblasts or stimulated peripheral blood lymphocytes after x-irradiation or fluorescent light exposure. Susceptibility to cancer is found when the frequency of breaks and gaps in the cell sample is two to three-fold higher than that occurring in comparable cells from control individuals. Various factors have been found which influence the accuracy of the test results. These factors include pH, temperature, cell density, culture medium or serum, microbial contamination and visible light exposure (effective wavelength 500 nm). Additionally, because of experimental variability, known normal controls are suggested for use in each test group.
REFERENCES:
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Obe, G., Beek, B., "The Human Leukocyte Test System" in Chemical Mutagens Principles & Methods for Their Detection, de Serres, F. J., & Hollaender, A., eds., Plenum Press, N.Y. and London, (1982), pp. 386-391.
Priest, Jean H., M.D.; "Medical Cytogenetics and Cell Culture" 268-269, pp. 60, 61, 102-107, 130-131, 292, 293, 296, 297, 298-303, 320-321, 324-327, Leu & Febiger (1977), Philadelphia.
Preston, J., "Cytogenic Abnormalities as an Indicator of Mutagenic Exposure" in Single-Cell Mutation Monitoring Systems, Ansari, A. A. and De Serres, F. J., eds., Plenum Press, N.Y. and London, pp. 127-143.
Mitchell, A. D.; Mirsalis, J. C.; "Unscheduled DNA Synthesis as an Indicator of Genotoxic Exposure" in Single-Cell Mutation Monitoring Systems, Ansari, A. A. and De Seres, F. J., eds., Plenum Press, NY and London, pp. 180-182.
Carrano, A. V.; Moore, II, D. H.; "The Rationale and Methodology for Quantifying Sister Chromatid Exchange in Humans" in Mutagenicity-New Horizons in Genetic Toxicology, Heddle, J. A., ed., Academic Press (1982), pp. 268-273.
Risley, M. S., "Mitotic Chromosomes", in Advanced Cell Biology, Schwartz, L. M. and Azar, M. M., eds., Van Nostrand Reinhold Company, (1981), pp. 1056-1059.
Penso, G.; Verani, P.; and Balducci, D.; "Nuclear Abnormalities in Irradiated Cultures: Relationship with those Reported in Virus Transformed Cells", in Cancer Cells in Culture, Katsuta, H., ed., University of Tokyo Press, (1968), pp. 205-215.
Jones Gary M.
Parshad Ram
Sanford Katherine K.
Nucker Christine M.
United States of America Represented by the Secretary of the Dep
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