Process for detecting enzymatically active xanthine oxidase

Details Process for detecting enzymatically active xanthine oxidase Process for detecting enzymatically active xanthine oxidase

1979-02-23

1981-01-20

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

C12Q 126

Patent

active

042463416

ABSTRACT:
A process for detecting and visualizing enzymatically active xanthine oxidase which comprises first analysis by discontinuous polyarcylamide gel electrophoresis and then staining the polyacrylamide gel containing xanthine oxidase using neotetrazolium chloride.

REFERENCES:
Anderson et al., Science, 120, 956, (1954).
Davis, Ann. N.Y. Acad. Sci., 121, 404-427, (1964).
Ornstein, Ann. N.Y. Acad. Sci., 121, 321-348, (1964).
Feinstein et al., Analytical Biochemistry, 56, 353-360, (1973).
Zittle et al., J. of Dairy Science, vol. XXXIX, No. 5, 522-527, (1956).
Owen, Methods in Enzymology, vol. XVIII, Part B, Academic Press, New York, Colowick, Editor, (1971), 590-592.

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