Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1996-03-08
1998-06-30
Gitomer, Ralph
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 29, C12Q 168, C12Q 102
Patent
active
057732191
ABSTRACT:
A process for aiding in the diagnosis of Alzheimer disease is disclosed in which the frequency of chromatid breaks and gaps is calculated in G.sub.1 -phase fluorescent-light-irradiated skin fibroblasts or stimulated peripheral blood lymphocytes after the addition of the DNA repair inhibitor caffeine or in G.sub.2 -phase fluorescent-light-irradiated skin fibroblasts or stimulated peripheral blood lymphocytes after the addition of the DNA repair inhibitor 1.beta.-D-arabinofuranosylcytosine (ara-C). In the G.sub.1 -phase test, the presence of Alzheimer disease is indicated when the total frequency of breaks and gaps caused by caffeine in the cells being examined is higher than the total frequency caused by caffeine in the normal cells. In the G.sub.2 -phase test, the presence of Alzheimer disease is indicated when the total frequency of breaks and gaps in the presence of ara-C is much less in the cells being examined than in normal cells.
REFERENCES:
patent: 4933274 (1990-06-01), Sandford et al.
Parshad R., Cytogenetic Evidence for a Cell Cycle Dependent DNA Repair Deficiciency: A Possible Diagnostic Feature of Alzheimer Disease, Proceedings of the 8th International Congress of Human Genetics, American J of Human Genetics Supp 49, 155, Oct. 1991.
Backon J., Dementia in Cancer Patients Undergoing Chemotherapy: Implication of Free Radical Injury and Relevance to Alzheimer Disease, Medical Hypothesis 35 146-147, 1991.
Parshad, R., Price, F. Tarone, R.J. Robbins and Sanford, K.K. (1991) "Cytogenetic evidence for a cell-cycle-dependent DNA repair deficiency: A possible diagnostic feature of Alzheimer Disease." Proceedings of the 8th International Congress of Human Genetics, 6-11 Oct. 1991, Washington, D.C. U.S.A., Am. J. Human Genetics Suppl. 49, 155.
Chen, P., et al., "Heterogeneity in Alzheimer's disease: evidence from cellular radiosensitivity and complementation of this phenotype", Mutation Res. (1991) 256, 21-7.
Tobi, S.E., et al. "Chromosomal radiosensitivity of lymphocytes from Alzheimer's disease patents", J. Med. Genet. (1990) 437-40.
Parshad, R., et al., "Susceptibility to fluorescent light-induced chromatid breaks associated with DNA repair deficiency and malignant transformation in culture", Cancer Res. (1980) 40, 4415-9.
Price, F.M., et al., "Radiation-induced chromatid aberratons in Cockayne syndrome and xeroderma pigmentosum group C fibroblasts in relation to cancer predisposition", Cancer Genet. Cytogenet. (1991) 57, 1-10.
Sanford K.K., et al., "Response of human cells in culture to hydrogen peroxide and related free radicals generated by visible light: relationship to cancer susceptibility", in Free Radicals, Aging, and Degenerative Diseases, edited by Johnson, J.E., et al., Alan R. Liss, Inc., New York, 1986, pp. 373-394.
Sanford, K.K., et al., "Role of DNA repair in malignant neoplastic transformation of human mammary epithelial cells in culture", Carcinogenesis (1992) 13, 1137-41.
Parshad, R., et al., "Neoplastic Transformation of human cells in culture associated with deficient repair of light-induced chromosomal DNA damage", Int. J. Cancer: 30, 153-159 (1982).
Parshad, R., et al., "Chromatid damage induced by fluorescent light during G.sub.2 phase in normal and Gardner Syndrome fibroblasts," Interpretation in terms of deficient DNA repair, Mutation Research, 151 (1985) 57-63.
Gantt R., et al., "Enhanced G.sub.2 chromatid radiosensitivity, an early stage in the neoplastic transformation of human epidermal keratinocytes in culture", Cancer Research 47, 1390-1397 (1987).
Parshad, R., et al., A DNA-Repair Defect In Alzheimer Disease, vol. 42, No. 1, 98A (1994) (Abstracts Submitted to the Annual Meeting of the Western Section of the American Federation for Clinical Research held Feb. 9-12, (1994) (published after filing date of original priority application).
Melnick, L., et al., "Defective DNA Repair In Alzheimer Disease: Use In A Predictive Test On Cultured Cell Lines", Clinical Research vol. 42, No. 3, 464A (Oct. 1994) (published after filing dates of priority applications).
Parshad, R., et al., "Defective DNA Repair In Sporadic, Familial, And Down-Syndrome Alzheimer Disease", Journal of Investigative Medicine, vol. 43 (Supplement 2), 415A (Apr. 1995) (published after filing dates of priority applications).
Melnick, L.K., et al., "Xeroderma Pigmentosum Group-A and Alzheimer Disease Cells Have Similar Defective Repair of Fluorescent Light-Induced DNA Damage", Journal of Investigative Medicine, vol. 43 (Supplement 3), 508A, (Sep. 1995) (published after filing dates of priority applications).
Parshad Ram
Robbins Jay H.
Sanford-Mifflin Katherine K.
Gitomer Ralph
The United States of America as represented by the Department of
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