Process for degradation of keratin, keratinaceous material, coll

Details Process for degradation of keratin, keratinaceous material, coll Process for degradation of keratin, keratinaceous material, coll

1993-03-08

1995-03-07

Naff, David M.

Drug, bio-affecting and body treating compositions

Enzyme or coenzyme containing

Hydrolases

424 9467, 435219, 435859, A61K 3754, C12N 950, C12N 100

Patent

active

053956133

ABSTRACT:
A process for the degradation of keratin, keratinaceous material, collagen or collagenaceous material comprising contacting keratin, keratinaceous material, collagen or collagenaceous material with an enzyme material isolated from a culture of Micrococcus sedentarius. The enzyme material comprises one or more proteases having the ability to degrade keratin, keratinaceous material, collagen or collagenaceous material. The enzyme material is water soluble, non-dialysable through a membrane having a molecular weight cut-off of 10 kDa, has an isoelectric point of 4.6 or 2.7, a molecular weight of 30.3 kDa or 50 kDa, an optimum pH for protease activity at about 8.2 or at about 10.2 and an optimum temperature for protease activity at about 40.degree. C. or 46.degree. C.

REFERENCES:
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K. M. Nordstrom et al., Similarities Between Dermatophilus congolensis and Micrococcus sedentarius, (vol. 87, No. 0, p. 244, 1987).
K. T. Holland et al., Qualitative and Quantitative Assay for Detection of Callus Degrading Activity by Bacteria, (vol. 11, No. 4, pp. 224-227, 1990).
K. T. Holland et al., The Effect of pH on the Growth of Proteinase Production of Micrococcus sedentarius in Relation to Pitted Keratolysis, (vol. 95, No. 4, p. 473, 1990.
Kloos, et al., Isolaiton and Characterization of Micrococci From Human Skin, Including Two New Species: Micrococcus lylae and Micrococcus kristinae, (Int'l Journal of Systematic Bacteriology, Jan. 1974, vol. 24, No. 1 pp. 70-101).
Laemmli, Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4, (Nature, Aug. 1970, vol. 227, pp. 680-685).
Millet, Characterization of Proteinases Excreted by Bacillus subtilis Marburg Strain during Sporulation, (Journal of Applied Bacteriology, 1970, 33, pp. 207-219).
Schleifer et al., The Genus Micrococcus, (A Handbook on Habitats, Isolation and Identification of Bacteria, vol. 2, pp. 1539-1547).
Winter et al., Analytical Electrofocusing in Thin Layers of Polyacrylamide Gels, (LKB Application Note 250, Dec. 1977).
Marshall et al., The Cutaneous Microbiology of Normal Human Feet, (Journal of Applied Bacteriology, 1987, vol. 62, pp. 139-146).
Nordstrom et al., Pitted Keratolysis, The Role of Micrococcus sedentarius, (Arch Dermatol-vol. 123, Oct. 1987, pp. 1320-1325).
Marshall et al., A Comparative Study of the Cutaneous Microflora of Normal Feet with Low and High Levels of Odour, (Journal of Applied Bacteriology, 1988, vol. 65, pp. 61-68).
Holland et al., Zentralbl. Bakteriol., 1989, (21 Suppl.), The Effect of Dilution Rate on . . . .

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