Process for decreasing the thermal stability of microbial rennet

Details Process for decreasing the thermal stability of microbial rennet Process for decreasing the thermal stability of microbial rennet

1979-09-10

1982-09-07

Naff, David M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

426 36, 426 63, 435931, C12N 958, A23C 19032

Patent

active

043484824

ABSTRACT:
A process is provided for decreasing the thermal stability of Mucor microbial rennet without substantially reducing its milk-clotting activity by contacting an aqueous solution thereof with a methionine-oxidizing means under conditions effective substantially to decrease the thermal stability of the microbial rennet. The resultant treated microbial rennet is dramatically less heat stable than untreated microbial rennet and can serve as replacement for calf rennet in traditional cheese-making processes. The preferred methionine-oxidizing means is hydrogen peroxide or dye-sensitized photooxidation.

REFERENCES:
patent: 2053740 (1936-09-01), Reichert et al.
patent: 2125398 (1938-08-01), Reichert et al.
patent: 3886288 (1975-05-01), Rice et al.
patent: 4136201 (1979-01-01), Feldman
patent: 4255454 (1981-03-01), Branner-Jorgensen
Ilany et al., Milk-Clotting Activity of Proteolytic Enzymes, J. Da. Sci., vol. 52, No. 1, 1969 (pp. 43-46).
Neumann, N. P., Oxidation with Hydrogen Peroxide, Methods in Enzymology, vol. XXV, 1972 (pp. 393-400).
Regmier, J. M., Thermal Resistance of Clotting Enzymes, Chemical Abstracts, vol. 87: 518172, 1977 (p. 352).
Iori et al., Dye-Sensitized Selective Photooxidation of Methionine, Biochemica et Biophysica ACTA, vol. 154, 1968 (pp. 1-9).
McBride-Warren et al., Structural and Functional Determinants of Mucor Miehei Protease, Biochimica et Biophysics ACTA, vol. 328, 1973 (pp. 52-60).

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