Process for culturing saffron stigma tissues

Chemistry: molecular biology and microbiology – Spore forming or isolating process

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4352404, 43524046, C12N 504

Patent

active

052178978

ABSTRACT:
This invention relates to a process for producing stigma-like tissues of saffron in a large scale by cutting the tissues of stigma (c), style (d, e, f), ovary (h, i, k), ovule (j), and petal (r) of saffron flower, and subculturing them on a liquid or solid LS medium or B5 medium containing a cytokinin and an auxin as main hormones.

REFERENCES:
Product Information, SAN-EI Chemical Industries, Ltd.
Handbook of Oxalox Co., Ltd. for "Purelox", pp. 1-11, and chart.
Report of the Society for Antibacterial and Antifungal Agents, Japan, pp. 1-4.
Himeino et al. (1987) Agric. Biol. Chem 51(9): 2395-2400.
Sano et al. (1987) Plant Cell, Tissue and Organ Culture 11: 159-166.
Evans, et al. (1981) in TA Thorpe, ed. Plant Tissue Culture, Academic Press, NY, pp. 45-53.
Toshiake Matsuzaki et al., Plant Biotechnology, "Method of In-vitro Cultivation of Stigma Tissues" published by Kabushiki Kaisha Tokyo Kagaku Dojin, Apr. 25, 1986.
I. K. Vasil "Cell Culture and Somatic Cell Genetics of Plants", vol. 1, pp. 221-226, Academic Press Inc. (1984).
I. K. Vasil "Cell Culture and Somatic Cell Genetics of Plants", vol. 1, pp. 18-22, Academic Press Inc. (1984).

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