Process for culturing hepatocytes for formation of spheroids

Details Process for culturing hepatocytes for formation of spheroids Process for culturing hepatocytes for formation of spheroids

1995-05-30

1997-04-29

Kunz, Gary L.

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Method of detaching cells, digesting tissue or establishing...

435402, 435395, 514 54, 514 56, 536 21, 536 53, 536 54, 536 55, 536 551, 536124, 5361231, C12N 500, A61K 31715, C07H 100

Patent

active

056248393

ABSTRACT:
Hepatocytes spheroids can be formed by culturing hepatocytes in a culture vessel using a lipid-bound glycosaminoglycan as a culture substrate. Floating spheroids of hepatocytes can be obtained efficiently, which are capable of maintaining liver-specific functions and of keeping the spheroid form stably for a prolonged period of time.

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