Chemistry: molecular biology and microbiology – Plant cell or cell line – per se ; composition thereof;... – Culture – maintenance – or preservation techniques – per se
Reexamination Certificate
2005-02-22
2005-02-22
Campell, Bruce R. (Department: 1654)
Chemistry: molecular biology and microbiology
Plant cell or cell line, per se ; composition thereof;...
Culture, maintenance, or preservation techniques, per se
Reexamination Certificate
active
06858430
ABSTRACT:
The present invention provides a tissue culture method for cultivation of marine algae, said method comprising the steps of (i) establishing axenic viable algal material by sequential treatment thereof in sterile sea water supplemented with domestic liquid detergent, incubating the treated material, (ii) culturing the axenic explants on agarified medium for induction of callus; (iii) excising and subculturing the calli from the axenic explants on fresh agar plates to obtain differentiated densely pigmented oval or spherical shaped micro-propagules (iv) subculturing the pigmented calli in agarified medium to achieve enhanced somatic embryogenesis and micro-propagule formation in pigmented filamentous callus, (v) transferring the filamentous calli with somatic embryos for morphogenesis and development of young plantlets; and (vi) cultivating algal biomass on a large scale by growing the young plantlets in enclosed perforated polythene bags.
REFERENCES:
Dawes, C.J. et al., “Branch, Micropropagule and tissue culture of the red algaeEucheuma denticulatumandKappaphycus alvarezzifarmed in the Philippines”, Kluwer Academic Publishers, J. of Applied Phycology 3: 247-257, 1991.*
Mairh, O.P. etal., “Culture of marine red algaKappphycus striatum(Schmitz) Doty on the Saurashtra region, west coast of India,” Indian Journal of Marine Sciences, vol. 24, Mar. 1995, 24-31.*
Campbell, N.A. et al., “Biology,” sixth edition, 2002, Benjamin Cummings, pp. 564 and 784.
Eswaran Kuruppan
Ghosh Pushpito Kumar
Kumar Guru Rajakrishna
Mairh Om Prakash
Mody Kalpana Harish
Campell Bruce R.
Council of Scientific and Industrial Research
McCormick-Ewoldt S B.
Merchant & Gould P.C.
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