Process for cross-linking hyaluronic acid to polymers

Details Process for cross-linking hyaluronic acid to polymers Process for cross-linking hyaluronic acid to polymers

2001-08-02

2004-03-09

Wu, David W. (Department: 1713)

Synthetic resins or natural rubbers -- part of the class 520 ser

Synthetic resins

Mixing of two or more solid polymers; mixing of solid...

C525S054200, C524S500000, C524S029000, C424S423000, C424S488000, C424S449000, C536S041000, C536S055100, C536S004100, C514S054000, C514S777000

Reexamination Certificate

active

06703444

ABSTRACT:

The present invention relates to a process for the production of hyaluronic acid (HA) derivatives, in particular multiple eg double cross-linked hyaluronic acid derivatives, and to novel cross-linked derivatives so obtained, to products containing them and their uses in cosmetic, medical and pharmaceutical applications.
HA is a member of a class of polymers known as glycosaminoglycans. HA is a long chain linear polysaccharide and is usually present as the sodium salt which has a molecular formula of (C
14
H
20
NNaO
11
)
n
where n can vary according to the source, isolation procedure and method of determination. However, molecular weights of up to 14×10
6
have been reported.
HA and its salts can be isolated from many sources including human umbilical cord, rooster combs and nearly all connective matrices of vertebrate organisms. HA is also a capsular component of bacteria such as Streptococci as was shown by Kendall et al, (1937),
Biochem. Biophys. Acta,
279,401-405; it may therefore also be obtained by fermentation methods. For example, the present applicant's U.S. Pat. No 5,411,874 describes a method for producing hyaluronic acid by continuous fermentation of
Streptococcus equi.
HA is non-immunogenic and therefore has great potential in medicine. Because of its visco-elastic properties HA having a high molecular weight (over 1 million) has been found to be particularly useful in a variety of clinical fields, including wound treatment, ophthalmic surgery and orthopaedic surgery. HA is also potentially useful in a variety of non-medical fields, such as cosmetic applications.
However, the use of HA in certain of these applications is limited by the fact that following administration to humans HA is readily degraded by enzymes such as hyaluronidases and free radicals. Furthermore, HA is soluble in water at room temperature, which can also make it less suited to certain applications. Various attempts have therefore been made to prepare more stable forms of HA, in particular by cross-linking the HA molecules.
Thus, U.S. Pat. No. 4,582,865 (Biomatrix Inc.) describes the preparation of cross-linked gels of hyaluronic acid which are formed by cross-linking HA either by itself or mixed with other hydrophilic polymers using divinyl sulfone as the cross-linking agent. It appears that in this case the cross-linking occurs via the hydroxyl groups of HA.
U.S. Pat. No. 5,550,187 (Collagen Corporation) describes a method for preparing cross-linked biomaterial compositions which involves mixing a biocompatible polymer, which is preferably collagen but may be selected from other polymers including hyaluronic acid, with a sterile dry cross-linking agent such as a synthetic hydrophilic polymer.
U.S. Pat. No. 5,510,121 (Rhee et al.) describes conjugates formed by covalently binding glycosaminoglycans or derivatives thereof to hydrophilic synthetic polymers, in particular activated PEG derivatives. The process described appears to involve only one cross-linking step.
U.S. Pat. No. 5,578,661 (Nepera Inc.) describes a gel forming system for use as a wound dressing which is formed from three main components, the first being a water soluble polymer, the second being an acid-containing polymer and the third being a polysaccharide or amino-containing polymer such as hyaluronic acid In this case the cross-linking appears to be via ion-bonding.
U.S. Pat. No. 5,644,049 (Italian Ministry for Universities and Scientific and Technology Research) describes a biomaterial comprising an inter-penetrating polymer network (IPN) wherein one of the polymer components is an acidic polysaccharide such as hyaluronic acid and the second polymer component may be a synthetic chemical polymer. The two components may be (but are not necessarily) cross-linked.
Tomihata and Ikada have reported cross-linking of HA using a water soluble carbodiimide as cross-linking agent. It was postulated that cross-linking took place via ester groups. The cross-linking reaction was also carried out in the presence of L-lysine methyl ester, which appeared to give additional cross-linking via amide bonds to the lysine ester. (J.Biomed.Mater.Res., 37,243-251,1997).
U.S. Pat. No 5,800,541 describes collagen-synthetic polymer matrices prepared using a multiple step reaction. The first step involves reacting collagen with a synthetic hydrophilic polymer, the resulting matrix may then be modified in a second reaction step which may involve cross-linking or conjugating the matrix with a synthetic polymer, coupling biologically active molecules or glycosaminoglycans to the matrix, cross-linking the matrix using conventional chemical cross-linking agents or modifying the collagen in the matrix by means of chemical reaction. In this process, the initial collagen-synthetic polymer matrix appears to be cross-linked via only one type of bond, and the additional process steps serve to introduce further chemical substances which may form different types of bonds. However, it does not appear that any two of the substances forming the product will be linked to each other by more than one type of bond.
International patent application WO 97/04012 (Agerup) describes polysaccharide (which may be inter alia hyaluronic acid) gel compositions which are prepared by forming an aqueous solution of the polysaccharide, initiating cross-linking in the presence of a polyfunctional cross-linking agent, sterically hindering the cross-linking reaction from being terminated before gelation occurs (eg by diluting the solution) and then reintroducing sterically unhindered conditions (eg by evaporating the solution) so as to continue the cross-linking to a viscoelastic gel. There is no suggestion in this application that different types of bonds are formed in the two cross-linking stages.
None of the aforementioned documents describe products in which HA is linked to one or more polymer molecules (which may be the same or different) by means of two different types of cross-linking bonds.
We have now found that hyaluronic acid may be cross-linked with other polymers by two different types of cross-linking bonds, to effect a ‘double cross-linking’. The formation of different types of bonds is achieved by effecting the cross-linking via different functional groups. The bonds so formed can therefore be described as functional bonds. Thus, for example one type of bond may be formed by cross-linking via hydroxyl groups and a different functional bond formed by cross-linking via e.g. carboxyl groups. Such multiple cross-linking has been found to result in a high degree of cross-linking with improved biostability of HA based on the degree of crosslinking and selection of the second polymer.
In a first aspect therefore, the present invention provides a process for the preparation of multiple (e.g. double) cross-linked derivatives of hyaluronic acid, which process comprises cross-linking HA to one or more polymers other than HA and optionally to another molecule of HA via two or more different functional groups.
The crosslinking of each type of functional group may be effected by contacting HA and at least one other polymer, with one or more cross-linking agents, simultaneously or sequentially, as described in more detail hereinbelow.
In this specification the polymer to which HA may be cross-linked will be referred to generally as “polymer”, “second polymer” or “subsequent polymer”. A “second polymer” or “subsequent polymer” may comprise a mixture of polymers.
In this specification, ‘multiple crosslinked HA’ refers to a hyaluronic acid derivative wherein a molecule of HA is cross-linked to a second polymer and optionally to a subsequent polymer and/or another molecule of HA by means of two or more different types of functional bond. Similarly, ‘double crosslinked HA’ refers to a hyaluronic acid derivative wherein a molecule of HA is cross-linked to a second polymer and optionally to a subsequent polymer and/or another molecule of HA by means of two different types of functional bond and ‘single crosslinked HA’ refers to a hyaluronic acid derivative wherein a molecule of H

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