Process for controlling cultures of recombinants

Details Process for controlling cultures of recombinants Process for controlling cultures of recombinants

1988-06-02

1997-10-07

Ketter, James

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435207, C12Q 168, C12N 938

Patent

active

056746784

ABSTRACT:
Expression of a desired gene and production of a product from the desired gene can be obtained effectively by cultivating a microorganism containing a hybrid plasmid comprising the desired gene, a vector and a promoter in its cell, and adding an inducer and a nutriment simultaneously to the culture medium at the time when the nutriment originally present in the culture medium is almost consumed.

REFERENCES:
patent: 3912585 (1975-10-01), Iijima et al.
patent: 4551433 (1985-11-01), DeBoer
patent: 4891310 (1990-01-01), Shimizu et al.
"High Density Cultivation of Biomass in Fed-Batch System with DO-Stat" by Mori, et al., Journal of Chemical Engineering of Japan, vol. 12, 1979, pp. 313-319.

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