Process for controlling contamination of nucleic acid amplificat

Details Process for controlling contamination of nucleic acid amplificat Process for controlling contamination of nucleic acid amplificat

1997-11-03

1999-08-31

Zitomer, Stephanie

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, 435195, 435810, 435194, 935 77, 935 78, C12P 1934, C12Q 168, C12N 914, C12N 912

Patent

active

059453133

ABSTRACT:
This invention relates to a method of incorporating an exo-sample nucleotide into the amplified product strands resulting from a nucleic acid amplification process. Once the product strands have been obtained and analyzed (e.g., by hybridization, Southern blot, etc.), the exo-sample strands can be selectively destroyed by acting on the incorporated exo-sample nucleotide. Two embodiments are presented. In a first embodiment, the exo-sample nucleotide is incorporated by carrying out the amplification reaction in the presence of an excess of exo-sample nucleotide triphosphate. In a second embodiment, the exo-sample nucleotide is incorporated by carrying out the amplification reaction in the presence of an oligonucleotide which has, as part of its sequence, one or more exo-sample nucleotides.

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