Process for controlling contamination of nucleic acid amplificat

Details Process for controlling contamination of nucleic acid amplificat Process for controlling contamination of nucleic acid amplificat

1994-04-01

1997-11-04

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 912, 435 6, 435200, 935 77, 935 78, C12P 1934, C12Q 168

Patent

active

056838964

ABSTRACT:
This invention relates to a method of incorporating an exo-sample nucleotide into the amplified product strands resulting from a nucleic acid amplification process. Once the product strands have been obtained and analyzed (e.g., by hybridization, Southern blot, etc.), the exo-sample strands can be selectively destroyed by acting on the incorporated exo-sample nucleotide.
Two embodiments are presented. In a first embodiment, the exo-sample nucleotide is incorporated by carrying out the amplification reaction in the presence of an excess of exo-sample nucleotide triphosphate.
In a second embodiment, the exo-sample nucleotide is incorporated by carrying out the amplification reaction in the presence of an oligonucleotide which has, as part of its sequence, one or more exo-sample nucleotides.

REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 4873192 (1989-10-01), Kunkel
patent: 4876187 (1989-10-01), Duck et al.
patent: 4965188 (1990-10-01), Mullis et al.
patent: 5035996 (1991-07-01), Hartley
patent: 5418149 (1995-05-01), Gelfand et al.
Almoguera, C., et al., "Most Human Carcinomas of the Exocrine Pancreas Contain Mutant c-K-ras Genes," Cell 53(4):549-554 (May 20, 1988).
Bos, J.L., et al., "Prevalence of ras Gene Mutations in Human Colorectal Cancers," Nature 327:293-297 (1987).
Engelke, D.R., et al., "Direct Sequencing of Enzymatically Amplified Human Genomic DNA," Proc. Natl. Acad. Sci. USA 85(2):544-548 (Jan. 1988).
Erlich, H.A., et al., "Specific DNA Amplification," Nature 331:461-462 (Feb. 4, 1988).
Farr, C.J., et al., "Analysis of RAS Gene Mutations in Acute Myeloid Luekemia by Polymerase Chain Reaction and Oligonucleotide Probes," Proc. Natl. Acad. Sci. USA 85(5):1629-1633 (Mar. 1988).
Higuchi, R., et al., "DNA Typing from Single Hairs," Nature 332:543-546 (Apr. 7, 1988).
Higuchi, R., et al., "A General Method of in vitro Preparation and Specific Mutagenesis of DNA Fragments: Study of Protein and DNA Interactions," Nucl. Acids Res. 16(15):7351-7367 (Aug. 11, 1988).
Kwok, S., et al., "Identification of Human Immunodeficiency Virus Sequences by Using In Vitro Enzymatic Amplification and Oligomer Cleavage Detection," J. Virol. 61(5):1690-1694 (1987).
Langer, P.R., et al., "Enzymatic Synthesis of Biotin-labeled Polynucleotides: Novel Nucleic Acid Affinity Probes," Proc. Natl. Acad. Sci. USA 78(11):6633-6637 (1981).
Lindahl, T., et al., "DNA N-Glycosidases. Properties of Uracil-DNA Glycosidase from Escherichia coli," J. Biol. Chem. 252:3286-3294 (1977).
Marx, J.L., "Multiplying Genes by Leaps and Bounds," Science 240:1408-1410 (Jun. 10, 1988).
Maxam, A.M., and Gilbert, W., "A New Method for Sequencing DNA," Proc. Natl. Acad. Sci. USA 74:560-564 (1977).
Mole, S.E., et al., "Using the Polymerase Chain Reaction to Modify Expression Plasmids for Epitope Mapping," Nucl. Acids Res. 17(8):3319 (Apr. 25, 1989).
Mullis, K.B., and Faloona, F.A., "Specific Synthesis of DNA in Vitro via a Polymerase-Catalyzed Chain Reaction," Meth. Enzymol. 155:335-350 (1987).
Orlandi, R., et al., "Cloning Immunoglobulin in Variable Domains for Expression by the Polymerase Chain Reaction," Proc. Natl. Acad. Sci. USA 86(10):3833-3837 (May 1989).
Oste, C., "Polymerase Chain Reaction," BioTechniques 6(2):162-167 (Feb. 1988).
Ou, C.-Y. et al., "DNA Amplification for Direct Detection of HIV-1 in DNA of Peripheral Blood Mononuclear Cells," Science 239:295-297 (Jan. 15, 1988).
Syvanen, A.-C., et al., "Quantification of Polymerase Chain Reaction Products by Affinity-based Hybrid Collection," Nucl. Acids Res. 16(23):11327-11338 (Dec. 9, 1988).
Vosberg, H.-P., "The Polymerase Chain Reaction: An Improved Method for the Analysis of Nucleic Acids," Hum. Genet. 83(1):1-15 (Aug. 1989).
Wong, C., et al., "Characterization of .beta.-Thalassaemia Mutations Using Direct Genomic Sequencing of Amplified Single Copy DNA," Nature 330:384-386 (1987).
Kornberg et al., DNA Replication, 136-137 (1992).
Barany, F., Genetic disease detection and DNA amplification using cloned thermostable ligase, Proc. Natl. Acad. Sci. USA 88:189-193 (1991).
Delort et al., Excision of uracil residues in DNA: mechanism of action of Escherichia coli and Micrococcus luteus uracil-DNA glycolases, Nuc. Acids Res. 13(2):319-335 (1985).
Doetsch et al., The enzymology of spurinic/apyrimidinic endonucleases, Mutation Research 236:173-201 (1990).
Duncan, B.K., DNA Glycosylases, The Enzymes XIV:565-586 (1981).
Cimino et al., More false-positive problems, Nature 345:773-774 (1990).
Kwoh et al., Transcription-based amplification system and detection of amplified human immunodeficiency virus type 1 with a bead-based . . . , Proc. Natl. Acad. Sci USA 86:1173-1177 (1989).
Kwok et al., Avoiding false positives with PCR, Nature 339:237-238 (1989).
Li et al., Amplification and analysis of DNA sequences in single human sperm and diploid cells, Nature 335:414-417 (1988).
Loh et al., Polymerase Chain Reaction with Single-Sided Specifity: Analysis of T Cell Receptor .delta.Chain, Science 243:217-220 (1989).
Mullis et al., Specific Enzyme Amplification of DNA In Vitro: The Polymerase Chain Reaction, Cold Spring Harbor Symposia on Quantitative Biology, vol. LI, pp. 263-273 (1986).
1990 Brochure from Perkin Elmer Corporation, "Gene AMP PCR Carryover Prevention Kit", B-3, p. 2 (1990).
European Search Report for EP 90309492 corresponding to U.S. application Ser. No. 7/401,840, filed Sep. 1, 1989.
European Search Report for EP 90306001 corresponding to U.S. application No. 07/360,120, filed Jun. 1, 1989.
Longo et al., Use of uracil DNA glycosylase to control carry-over contamination in polymerase chain reactions, Gene 93:125-128 (1990).
Saiki et al., Enzymatic Amplification of .beta.-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell Anemia, Science 230:1350-1354 (1985).
Saiki et al., Primer-Directed Enzymatic Amplification on DNA with Thermostable DNA Polymerase, Science 239:487-491 (1988).
Schaaper et al., Infidelity of DNA synthesis associated with bypass of apurinic sites, Proc. Natl. Acad. Sci. USA 80:487-491 (1983).
Varshney et al., Specificities and Kinetics of Uracil Excision from Uracil-Containing DNA Oligomers by Escherichia coli Uracil DNA Glycosylase, Biochem. 30:4055-4061 (1991).
Wu et al., The Ligation Amplification Reaction (LAR)--Amplification of Specific DNA Sequences Using Sequential Rounds of Tamplate-Dependent Ligation, Genomics 4:560-569 (1989).

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