Process for continuously culturing adherent animal cells

Details Process for continuously culturing adherent animal cells Process for continuously culturing adherent animal cells

1991-07-15

1993-06-15

Lacey, David L.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

435 703, C12N 508, C12P 2100

Patent

active

052197522

ABSTRACT:
Adherent 293 human kidney cells are continuously cultured in suspension in serum-free medium. Fresh medium is fed into a culture vessel and spent medium is withdrawn from the vessel. The 293 cells are maintained in small aggregates in suspension at a density of at least 3.times.10.sup.6 cells/ml for 30 days. The formation of large cell clumps is prevented by maintaining the concentration of calcium in the serum-free medium at 0.002 mM to 0.3 mM.

REFERENCES:
patent: 4059485 (1977-11-01), Tolbert et al.
patent: 4184916 (1980-01-01), Tolbert et al.
patent: 4560655 (1985-12-01), Baker
patent: 5114855 (1992-05-01), Hu et al.
Eagle, Amino Acid Metabolism in Mammalian Cell Culture Science, vol. 130, pp. 432-437 1959.
Nagafuchi, et al., Transformation of Cell Adhesion Properties by Exogenously Introduced E-Cadherin cDNA, Nature, vol. 329, pp. 341-343 24 Sep. 1987.

Affiliated with

Also associated with

Rating

Process for continuously culturing adherent animal cells does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Process for continuously culturing adherent animal cells, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for continuously culturing adherent animal cells will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1042013