Process for continuous optimized protein production in insect la

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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435 696, 435 6552, 435 691, C12P 2106, C12P 2104

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active

061534099

ABSTRACT:
The present invention provides for a recombinant insect larvae and a process of manufacturing proteins utilizing insect larvae that allows for the selection of individual larvae for harvest at the point of their optimal expression of a protein of interest. This invention also provides for a process to manufacture proteins in larvae that does not require synchronization of the infection, growth and harvest larvae to optimally manufacture a protein of interest. The invention further provides for a process of producing interleukin-2 in larvae.

REFERENCES:
patent: 5427858 (1995-06-01), Attie
patent: 5491084 (1996-02-01), Chalfie
Kuhn S and Zipfel PF. The baculovirus expression vector pBSV-His directs secretion of histidine-tagged proteins. Gene 162: 225-229, 1995.
Chao Y, Chen S, Li C. Pest control by fluorescence.Nature 380:396-397, Apr. 1996.
Bei R, Schlom J, and Kashmiri SVS. Baculovirus expression of a functional single-chain immunoglobulin and its IL-2 fusion protein. Journal of Immunological Methods 186:245-255, 1995.
Matsumoto T, Gargosky SE, Kelley K and Rosenfeld RG. Characterization of an insulin-like growth factor binding protein-5 protease produced by rat articular chondrocytes and a neuroblastoma cell line. Growth Regulation 6:185-190, Sep. 1996.
Chalfie et al., "Green Fluorescent Protein as Marker for Gene Expression", Science, 263:802-806 (1994).
Eriksson et al., "Green Fluorescent protein as tool for screening recombinant baculovirus", J. Virological Meth., 59:127-133 (1996).
Matsumura et al., "DNA shuffling brightens prospects for GFP", Nature Biotech, 14:366 (1996).
Miyajima et al., "Use of silkworm, Bombyx mori and an insect baculovirus vector for high level expression and secretion of biologically active mouse interleukin-3", Gene, 58:273-281 (1987).
Medin et al., "Efficient, low cost protein factories: Expression of human adenosine aminase in baculovirus infected insect larvae", PNAS, 87:2760-2764 (1990).
Niedz et al., "Green Fluorescent protein: An in vivo reporter of plant gene expression", Plant Cell Reports, 14:403-406 (1995).
Oker-Blom et al., "Highly efficient production of GFP and its derivatives in insect cells for visual in vitro applications", FEBS Lett., 389:238-243 (1996).
Pines et al., "GFP in mammalian cells", Trends Genet., 11(8):326-327 (1995).
Yeh et al., "Green Fluorescent protein as vital marker and reporter of gene expression in Drosophila", PNAS, 92:7036-7040 (1995).

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