Multicellular living organisms and unmodified parts thereof and – Method of using a transgenic nonhuman animal to manufacture...
Reissue Patent
2006-09-05
2006-09-05
Priebe, Scott D. (Department: 1633)
Multicellular living organisms and unmodified parts thereof and
Method of using a transgenic nonhuman animal to manufacture...
C435S069100, C435S069520, C435S069600, C435S069700, C800S013000
Reissue Patent
active
RE039270
ABSTRACT:
The present invention provides for a recombinant insect larvae and a process of manufacturing proteins utilizing insect larvae that allows for the selection of individual larvae for harvest at the point of their optimal expression of a protein of interest. This invention also provides for a process to manufacture proteins in larvae that does not require synchronization of the infection, growth and harvest larvae to optimally manufacture a protein of interest. The invention further provides for a process of producing interleukin-2 in larvae.
REFERENCES:
patent: 5472858 (1995-12-01), Attie et al.
patent: 5491084 (1996-02-01), Chalfie et al.
Kuhn S and Zipfel PF. The baculovirus expression vecotr pBSV-His directs secretion of histidine-tagged proteins, Gene 162: 225-229, 1995.
Chao Y, Chen S, Li C. Pest control by fluorescence, Nature 380:396-397, Apr. 1996.
Bei R, Schlom J, and Kashmiri SVS. Baculovirus expression of a functional single-chain immunglobulin and its IL-2 fusion protein. Journal of Immunological Methods 186:245-255, 1955.
Matsumoto T, Gargosky SE, Kelley K and Rosenfeld RG. Characterization of an insulin-like growth factor binding protein-5 protease, produced by rat articular chondrocytes and a neuroblastoma cell line, Growth Regul. 6:185-190, Sep. 1996.
Chalfie et al., “Green Fluorescent Protein as Marker for Gene Expression”, Science, 263:802-806 (1994).
Eriksson et al., “Green Fluorescent protein as a tool for screening recombinant baculovirus,” J. Virological Meth. 59:127-133 (1996)
Matsumura et al., “DNA shuffling brightens prospects for GFP”, Nature Biotech. 14:366 (1996)
Miyajima et al., “Use of silkworm, Bombyx mori and an insect baculovirus vector for high level expression and secretion of biologically active mouse interleukin-3”, Gene, 58:273-281 (1987).
Medin et al., “Efficient, low cost protein factories: Expression of human adenosine aminase in baculovirus infected insect larvae”, PNAS, 87:2760-2764 (1990).
Niedz et al., “Green Fluorescent protein: An in vivo reporter of plant gene expression”, Plant Cell Reports, 14:403-406 (1995).
Oker-Blum et al., “Highly efficient production of GFP and its derivatives in insect cells for visual in vitro applications”, FEBS Lett., 389:238-243, 1996.
Pines et al., “GFP in mammalian cells”, Trends Genet., 11(8):326-327 (1995).
Yeh et al., “Green fluorescent protein as a vital marker and reporter of gene expression in Drosophila”, Proc. Natl. Acad. Sci. USA 92:7036-7040, (1995).
Medin, J. A., Hung, L., Gathy, K., Evans, R.K., Coleman, M.S. 1990. Efficient, low-cost protein factories expression of human adenosine deaminase in baculovirus-infected insect larvae. Proc. Natl. Acad. Sci. USA 87: 2760-2764.
Naggie, S., Pulliam, T.R. Bentley, W.E. 1996. Elicitation of protease activity in insect (Sf-9) cell culture. Abstracts of the American Chemical Society 211: 163.
Niedz, R.P., Sussman, M.R., Satterlee, J.S. 1995. Green fluorescent protein: an in vivo reporter of plant gene expression. Plant Cell Reports 14: 403-406.
Ohkawa, T.K., Majima, S. Maeda. 1994. A cystein protease encoded by the baculovirusBombyx morinuclear polyhedrosis virus. J. Virol. 68: 6619-6625.
Page, A.P., and R.M. Maizels. Biosynthesis and glycosylation of serine/threonine-rich secreted proteins fromToxocara canislarvae, Parasitology, 1992, 105:297.
Palhan, V.B., Sumathy, S., Gopinathan, K.P. 1995. Baculovirus mediated high-level expression of luciferase in silkworm cells and larvae. BioTechniques 19: 97-104.
Pham, M.Q., Wier, M., Cha, H.J., Bentley, W.E. 1997. Human Interleukin-2 Production in Insect (Trichoplusia ni) Larvae: The Effects and Partial Control of Proteolysis, submitted in Biotechnology and Bioengineering.
Pines, J. 1995. GFP in mammalian cells. Trends Genet. 11: 326-327.
Prasher, D.C., Eckenrode, V.K., Ward, W.W., Prendergast, F.G., Cormier, M.J. 1992. Primary structure of theAequorea Victoriagreen fluorescent protein. Gene 111: 229-233.
Reilander, H., Haase, W., Maul, G. 1996. Functional expression of theAequorea Victoriagreen fluorescent protein in insect cells using the baculovirus expression system. Biochem. Biophys. Res. Commun. 219: 14-20.
Schlenstedt, G., Saavedra, C., Loeb, J.D.J., Cole, C.N., Silver, P.A. 1995. The GFP-bound form of the yeast Ran/TC4 homologue blocks nuclear protein import and appearance of poly(a)+RNA in the cytoplasm. Proc. Natl. Acad. Sci. USA 92: 225-229.
Shimomura, O., Johnson, F.H., Saiga, Y. 1962. Excitation, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan. Aequorea. J. Cell. Comp. Physiol. 59: 223-229.
Slack, J.M., J. Kuzio, P. Falkner. 1995. Characterization of n-cath, a cathepsin L-like proteinase expressed by the baculovirusAutographa californicanuclear polyhedrosis virus. J. Gen. Virol. 76: 1091-1098.
Stearns, T. 1995. The green revolution. Current Bio. 5:262-264.
Tamiguchi et al. 1983. Structure and Expression of Cloned cDNA for Human IL-2. Nature 302:305-310.
Tanada, Y. (1989). InInvertebrate Cell System Applications. vol. 2 (ed. Mitsuhashi). CRC Press, Boca Raton, FL., pp. 43-49.
Wu, G.-I., Zou, D.-J., Koothan, T., Cline, H.T. 1995. Infection of frog neurons vaccinia virus permits in vivo expression of foreign proteins. Neuron 14:681-684.
Bokman, S.H., Ward, W.W. 1981. Renaturation of Aequorea green-fluorescent protein. Biochem. Biophys. Res. Comm. 101: 1372-1380.
Cha, H.J., Pham, M.Q., Rao, G., Bentley, W.E. 1997. Expression of Green Fluorescent Protein in Insect Larvae and Its Application for Heterologous Protein Production, accepted in Biotechnology and Bioengineering., vol. 56, Iss. 3, 1997, 239-247.
Chalfie, M., Tu, Y., Euskirchen, G., Ward, W.W. Prasher, D.C. 1994. Green fluorescent protein as a marker for gene expression. Science 263: 802-805.
Crameri, A., Whitehorn, E.A., Tate, E., Stemmer, W.P.C. 1996. Improved green fluorescent protein by molecular evolution using DNA shuffling. Nature Biotechnol. 14;315-319.
Davis, T.R., and H.A. Wood. 1995. Intrinsic Glycosylation Potentials of Insect Cell Cultures and Insect Larvae, In vitro cellular & developmental biology journal. 31:659.
Gillis, S., Ferm, M.M., Ou, W., Smith, K.A. 1978. T cell growth factor: parameters of production and a quantitative microassay for activity. J. Immunol. 120:2027-2032.
Harcum, S.W., Bentley, W.E. 1993. Detection, quantification, and characterization of protease in recombinantEscherichia coli. Biotechnol. Techniques 7: 441-447.
Heinlein, M., Epel, B.L., Padgett, H.S., Beachy, R.N. 1995. Interaction of tobamovirus movement proteins with the plant cytoskeleton. Science 270: 1983-1985.
Ikawa, M., Kominami, K., Yoshimura, Y., Tanaka, K., Nishimune, Y., Okabe, M. 1995. Green fluorescent protein as a marker in transgenic mice. Devel. Growth. Differ. 37: 455-459.
Inouye, S., Tsuji, F.I. 1994. Aequorea green fluorescent protein: expression of the gene and fluorescent characteristics of the recombinant protein. FEBS Letters 341: 277-280.
Johnson, F.H., Shimomura, O., Saiga, Y., Gershman, L.C., Reynolds, G.T., Waters, J.R. 1962. Quantum efficiency of Cypridina luminescence, with a note on that of Aequorea. J. Cell. Comp. Physiol. 60: 85-103.
Kain, S.R., Adams, M., Kondepudi, A., Yang, T.T., Ward, W.W., Kitts, P. 1995) The green fluorescent protein as a reporter of gene expression and protein localization. BioTechniques 17: 982-987.
Kashima et al., 1985. Unique Structure of Murine IL-2 as Deduced from Cloned cDNA. Nature 313: 402.
Maeda, S., Kawai, T., Obinata, M., Fujiwara, H., Horiuchi, T., Saeki, Y., Sato, Y., Furusawa, M. 1985. Production of human α-interferon in silkworm using a baculovirus vector. Nature 315: 592-594.
Marumoto, Y., Sato, Y., Fujiwara, H., Sakano, K., Sakei, Y., Agata, M., Furusawa, M., Maeda, S. 1987. Hyperproduction of polyhedrin-IGF II fusion protein in silkworm larvae infected with recombinantBombyx morinuclear polyhedrus virus. J. Gen. Virol. 68: 2599-2606.
Miyajima, A., Schreurs, J., Otsu, K., Kondo, A., Arai, K., Maed
Bentley William E.
Cha Hyung Joon
Khanna Rita
Pham Minh-Quan
Hultquist Steven J.
Intellectual Property / Technology Law
Priebe Scott D.
University of Maryland Biotechnology Institute
LandOfFree
Process for continuous optimized protein production in... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Process for continuous optimized protein production in..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for continuous optimized protein production in... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3547116