Process for conducting electrophoresis and transfer

Details Process for conducting electrophoresis and transfer Process for conducting electrophoresis and transfer

1986-09-25

1988-07-12

Niebling, John F.

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

204299R, G01N 2726

Patent

active

047568097

ABSTRACT:
An apparatus for carrying out horizontal gel electrophoresis for separation and subsequent vacuum-assisted transportation of macromolecules to a support membrane to facilitate detection. The entire procedure is conducted in one cartridge. A method for conducting electrophoresis and subsequent vacuum-assisted transfer using the apparatus of the present invention is also disclosed.

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patent: 4541910 (1985-09-01), Davis, III et al.
patent: 4589965 (1986-05-01), Kreisher
E. M. Southern, "Detection of Specific Sequences Among DNA Fragments Separated by Gel Electrophoresis" Journal of Molecular Biology (1925) pp. 503-517.
M. Bittner et al., "Electrophoretic Transfer of Proteins and Nucleic Acids from Slab Gels to Diazobenzyloxymethyl Cellulose or Nitrocellulose Sheets"; Analytical Biochemistry 102, pp. 451-471 (1980).
Bethesda Research Laboratories, Inc., "Catalogue & Reference Guide" Aug. 1983, p. 51.
M. Peferon et al., "Vacuum-Blotting: A New Simple and Efficient Transfer of Proteins from Sodium Dodecyl Sulfate-Polyacrylamide Gels to Nitrocellulose" FEBS Letters, 1982, vol. 145, No. 2, pp. 369-371.
Zaitsev, I. Z. et al., "Vacuum Transfer of DNA to Filters for Detecting Inter-Individual Polymorphism by the Southern Blotting Hybridization Method" Byull. Eksp. Biol. Med., 1983, 96(10) pp. 84-86 (Abstract only).

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