Process for conducting electrophoresis and transfer

Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge

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204299R, G01N 2728, G01N 2726

Patent

active

048490784

ABSTRACT:
An apparatus for carrying out horizontal gel electrophoresis for separation and subsequent vacuum-assisted transportation of macromolecules to a support membrane to facilitate detection. The entire procedure is conducted in one cartridge. A method for conducting electrophoresis and subsequent vacuum-assisted transfer using the apparatus of the present invention is also disclosed.

REFERENCES:
patent: 4622124 (1986-11-01), Kreisher et al.
M. Peferoen et al., "Vacuum-Blotting: A New Simple and Efficient Transfer of Proteins from Sodium Dodecyl Sulfate-Polyacrylamide Gels to Nitrocellulose", FEBS Letters, vol. 145, No. 2.

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