Process for conducting biochemical reactions

Details Process for conducting biochemical reactions Process for conducting biochemical reactions

1989-10-18

1994-09-13

Housel, James C.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

43524025, 4352404, 43524046, 435243, 435285, 435286, 435287, 435311, 435313, 435315, 435316, C12N 500, C12N 502, C12M 112

Patent

active

053468260

ABSTRACT:
A process for growing procaryotic or eukaryotic cellular material in a tubular membrane immersed in an aqueous medium. The tubular membrane is filled with at least 10.sup.2 cells per milliliter at start-up and then maintained under controlled conditions to promote rapid growth of the cells so that the doubling time is less than 12 hours. A primary nutrient solution is continuously fed to the growing cells while some of the cells are being continuously withdrawn from the tubular membrane. The rate of cell removal is controlled so that that number of cells in the tubular membrane remains essentially constant at from 10.sup.2 to 10.sup.10 cells per milliliter and the production of metabolite byproduct remains the same under equilibrium conditions. Both cell material and metabolite byproducts are recycled.

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