Process for concentrating and separating trypsin inhibitor and k

Details Process for concentrating and separating trypsin inhibitor and k Process for concentrating and separating trypsin inhibitor and k

1987-10-02

1988-10-25

Therkorn, Ernest G.

Liquid purification or separation

Processes

Liquid/liquid solvent or colloidal extraction or diffusing...

210656, 435226, 435814, 435815, B01D 1508, C12N 964

Patent

active

047802090

ABSTRACT:
Two components, trypsin and kallidinogenase, in human urine are concentrated simultaneously by allowing human urine at neutral pH, collecting bubbles thus formed to obtain the concentrate of the two components, adjusting the concentrate to weak acidity, contacting the acidified concentrate with chitosan to allow the two components to be adsorbed onto chitosan, eluting the components from the adsorbent with aqueous ammonia solution, and neutralizing and heating the eluate at about 60.degree. C. for about 10 hours to make the eluate virus-free, followed by separating the components from the eluate.

REFERENCES:
patent: 4510248 (1985-04-01), Nakanishi
Carlson, Enzyme, 18:176 (1974).
Sumi et al., Journal of Physiol. of Japan, 39:53 (1977).
Johnson et al., Hoppe Seyler's Z. Physiol. Chem., 363:1167 (1982).
N. R. Shulman, J.B.C., 213:655 (1955).
T. A. Strup, Scan. J. Clin. Lab. Invest., 11:181 (1959).
G. J. Proksch, J. Lab. & Clin. Med., 79:491 (1972).
Sumi et al., Medicine and Biology, 100(1):37-39 (1980).
Celander et al., Arch. Biochem. Biophys., 55:286 (1955).

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