Process for cell disruption

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Lysis of micro-organism

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435267, 435274, 435874, 435101, 435104, C12N 106, C07G 1700, C13L 300, C12R 138

Patent

active

044812949

ABSTRACT:
Process for disrupting cells by contacting an aqueous, cell-containing medium with a protease enzyme, wherein the enzymic contact is preceded by contact with an ionic surfactant; the polysaccharide solutions thereby produced; and a process for displacing a fluid through a well and/or a permeable subsurface formation communicating with the well, by injecting into the well an optionally diluted, aqueous solution of such a polysaccharide.

REFERENCES:
patent: 3598181 (1971-08-01), Wegner et al.
patent: 3966618 (1976-06-01), Colegrove
patent: 4165257 (1979-08-01), Stokke
Birnboim et al., Nuclei Acids Res. vol. 7, #6, 1979, pp. 1513-1523.
"Methods in Microbiology", Ed. Norris and Ribbons, Publ. Academic Press, London and New Yori, 5B, pp. 371-372 (1971).

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