Process for binding IgG to protein A

Details Process for binding IgG to protein A Process for binding IgG to protein A

1984-06-22

1987-11-03

Nucker, Christine M.

Chemistry: analytical and immunological testing

Biospecific ligand binding assay

435 7, 436529, 436530, 436532, 436548, 436828, 530387, 530402, 530413, 530825, 935108, G01N 33566, G01N 33577, G01N 33544, G07K 312

Patent

active

047043660

ABSTRACT:
Unusually strong binding of IgG to protein A is achieved by contacting these components in the presence of a medium containing a high concentration of salt. Although such binding is of general utility, a particularly useful application is the purification of monoclonal antibodies from ascites fluid by affinity chromatography.

REFERENCES:
patent: 4430318 (1984-02-01), Langone
Lindmark et al., J. Immunol. Meth., 62 (1983) 1-13.
Hurrell, ed., Monoclonal Hybridoma Antibodies; Techniques and Applications, CRC Press, Inc., Boca Raton, Fla., 1982, pp. 51-52.
Lehninger, Biochemistry, Worth Publishers, Inc., N.Y., 1970, pp. 133-134.

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