Process for bacterial production of polypeptides

Details Process for bacterial production of polypeptides Process for bacterial production of polypeptides

1999-10-21

2010-12-28

Fronda, Christian L (Department: 1652)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

C435S183000, C435S200000, C435S201000, C435S252330, C435S320100, C536S023200

Reexamination Certificate

active

07858339

ABSTRACT:
Refractile particles containing a heterologous polypeptide as an insoluble aggregate are recovered from bacterial periplasm. The process involves culturing bacterial cells so as to express nucleic acid encoding phage lysozyme and nucleic acid encoding the heterologous polypeptide under separate promoters, disrupting the cells mechanically to release the phage lysozyme so as to release refractile particles from the bacterial cellular matrix, and recovering the released refractile particles from the periplasm. Chloroform is not used in any step and the recovery step minimizes co-recovery of cellular debris with the released refractile particles.

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