Process for bacterial production of polypeptides

Details Process for bacterial production of polypeptides Process for bacterial production of polypeptides

1996-10-31

1998-08-04

Kemmerer, Elizabeth C.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4352523, 43525233, 935 11, 935 43, C12N 1563

Patent

active

057891990

ABSTRACT:
A process is provided for producing a heterologous polypeptide in bacteria. This process comprises, in a first step, culturing bacterial cells that lack their native pstS gene and comprise nucleic acid encoding a PstS variant having an amino acid variation within the phosphate-binding region of the corresponding native PstS, nucleic acid encoding a DsbA or DsbC protein, nucleic acid encoding the heterologous polypeptide, a signal sequence for secretion of both the DsbA or DsbC protein and the heterologous polypeptide, an inducible promoter for the nucleic acid encoding the DsbA or DsbC protein, and an alkaline phosphatase promoter for the nucleic acid encoding the heterologous polypeptide. The nucleic acid encoding a PstS variant is under the transcriptional control of the wild-type pstS gene promoter. The second step of the process involves recovering the heterologous polypeptide from the periplasm or the culture medium.

REFERENCES:
patent: 5270181 (1993-12-01), McCoy et al.
patent: 5292646 (1994-03-01), McCoy et al.
patent: 5304472 (1994-04-01), Bass et al.
Bardwell et al., "The Bonds That Tie: Catalyzed Disulfide Bond Formation" Cell 74:769-771 (1993).
Bardwell et al., "Identification of a Protein Required for Disulfide Bond Formation In Vivo" Cell 67:581-589 (1991).
Bardwell et al., "A pathway for disulfide bond formation in vivo" Proc. Natl. Acad. Sci 90:1038-1042 (1993).
Bulleid, Neil J., "Protein Disulfide-Isomerase: Role in Biosynthesis of Secretory Proteins" Adv. Prot. Chem. 44:125-150 (1993).
Chivers et al., "The CXXC Motif: Imperatives for the Formation of Native Disulfide Bonds in the Cell" The EMBO Journal 15(11):2659-2667 (1996).
Creighton et al., "Catalysis by Protein-disulphide Isomerase of the Unfolding and Refolding of Proteins with Disulphide Bonds" J. Mol. Biol. 142:43-62 (1980).
Freedman et al., "Role of Protein Disulphide-Isomerase in the Expression of Native Proteins" Biochem. Soc. Symp. 55:167-192 (1989).
Grauschopf et al., "Why is DsbA Such an Oxidizing Disulfide Catalyst?" Cell 83:947-955 (1995).
Kamitani et al., "Identification and characterization of an Escherichia coli gene required for the formation of correctly folded alkaline phosphatase, a periplasmic enzyme" The EMBO Journal 11(1):57-62 (1992).
Knappik et al., "The Effect of Folding Catalysts on the In Vivo Folding Process of Different Antibody Fragments Expressed in Escherichia coli " Bio/Technology 11:77-83 (1993).
LaMantia et al., "The Essential Function of Yeast Protein Disulfide Isomerase Does Not Reside in Its Isomerase Activity" Cell 74:899-908 (1993).
Martin et al., "Crystal structure of the DsbA protein required for disulphide bond formation in vivo" Nature 365:464-468 (1993).
Missiakas et al., "The Escherichia coli dsbC (xprA) gene encodes a periplasmic protein involved in disulfide bond formation" The EMBO Journal 3(8):2013-2020 (1994).
Missiakas et al., "Identification and characterization of the Escherichia coli gene dsbB, whose product is involved in the formation of disulfide bonds in vivo" Proc. Natl. Acad. Sci. 90:7084-7088 (1993).
Noiva et al., "Protein Disulfide Isomerase" Journal of Biological Chemistry 267(6):3553-3556 (1992).
Shevchik et al., "Characterization of DsbC, a periplasmic protein of Erwinia chrysanthemi and Escherichia coli with disulfide isomerase activity" The EMBO Journal 13(8):2007-2012 (1994).
Wulfing et al., "Correctly Folded T-cell Receptor Fragments in the Periplasm of Escherichia coli " J. Mol. Biol. 242:655-669 (1994).
Wulfing et al., "Protein folding in the periplasm of Escherichia coli " Molecular Microbiology 12(5):685-692 (1994).
Wunderlich et al., "In Vivo Control of Redox Potential During Protein Folding Catalyzed by Bacterial Protein Disulfide-isomerase (DsbA)" Journal of Biological Chemistry 268(33):24547-24550 (1993).
Wunderlich et al., "Redox properties of protein disulfide isomerase (DsbA) from Escherichia coli " Protein Science 2:717-726 (1993).
Zapun et al., "The Reactive and Destabilizing Disulfide Bond of DsbA, a Protein Required for Protein Disulfide Bond Formation in Vivo" Biochemistry 32:5083-5092 (1993).

Affiliated with

Also associated with

Rating

Process for bacterial production of polypeptides does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Process for bacterial production of polypeptides, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for bacterial production of polypeptides will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1175855