Process for assaying the activity of tissue plasminogen activato

Details Process for assaying the activity of tissue plasminogen activato Process for assaying the activity of tissue plasminogen activato

1983-05-06

1986-01-07

Rosen, Sam

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving blood clotting factor

C12Q 156

Patent

active

045634203

ABSTRACT:
The invention relates to a process for assaying the activity of tissue plasminogen activator (TA), in which a sample is incubated with plasminogen, a substrate for plasmin giving a detectable reaction product on reaction with plasmin, preferably H-D-Val-Leu-Lys-pNA, and, as a stimulator for the conversion of plasminogen to plasmin by TA, water-soluble fibrinogen or fibrin fragments comprising at least partially the D-domains of the fibrinogen or fibrin molecule. The amount of the detectable reaction product of the plasmin substrate is measured. Preferably, said fibrinogen or fibrin fragments have been obtained by reacting fibrinogen or fibrin with CNBr and removing lower molecular components from the reaction product.
The invention also relates to kits for performing the assay and comprising the reagents to be used.

REFERENCES:
patent: 4011142 (1977-03-01), Jacobi
patent: 4278762 (1981-07-01), Svendsen

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