Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...
Patent
1992-03-06
1995-04-18
Schain, Howard E.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Blood proteins or globulins, e.g., proteoglycans, platelet...
530363, 530381, 530413, 530416, C07K 1506, C07K 318, C07K 328
Patent
active
054080398
ABSTRACT:
The invention relates to a process for purifying human von Willebrand factor from a cryoprecipitated plasma fraction, which comprises a combination of three chromatographic separation steps. The first chromatographic separation step comprises contacting a cryoprecipitated fraction with a large-pore vinyl polymer resin having DEAE group. The effluent from this separation step is again contacted with a large pore vinyl polymer resin having DEAE groups in the second chromatographic step. In the third chromatographic separation step, the effluent from the second step is subjected to affinity chromatography by contacting with gelatin-Sepharose. The concentrate obtained has very high specific activity and a high percentage of high molecular weight multimers. The concentrate is intended, in particular, for therapeutic use.
REFERENCES:
patent: 4728594 (1981-07-01), Amrani
patent: 4970300 (1990-11-01), Fulton et al.
patent: 5006642 (1991-04-01), Newman et al.
Vox Sanguinis, vol. 60, No. 1, (1991) pp. 8-15, T. Burnouf et al: `A highly purified factor VIII;c Concentrate prepared from cryoprecipitate by . . . .
Chemical Abstracts, vol. 96, No. 19, (1982), Abstract No. 158527, p. 379.
Search Report.
Burnouf Thierry
Burnouf-Radosevich Miryana
Centre Regional de Transfusion Sanguine de Lille
Schain Howard E.
Touzeau P. Lynn
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