Process for amplifying nucleic acids using DNA/PNA primers

Details Process for amplifying nucleic acids using DNA/PNA primers Process for amplifying nucleic acids using DNA/PNA primers

1997-09-11

2000-05-16

Horlick, Kenneth R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 911, 435 912, C12Q 168, C12P 1934

Patent

active

060635711

ABSTRACT:
The present invention relates to a novel advantageous process for amplifying nucleic acids using DNA/PNA primers and a temperature-stable polymerase enzyme.

REFERENCES:
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Carlsson, C., et al, "Screening for genetic mutations," Nature (1996) 380:207-210.
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Joyce, C.M., et al., "Function and Structure Relationships in DNA Polymerases," Annu. Rev. Biochem. (1994) 63:777-822.
Kong, H., et al., "Characterization of a DNA Polymerase from the Hyperthermophile Archaea Thermococcus litoralis, " J. Biol. Chem. (1993) 268:1965-1975.
Reyes, R.A., et al., "Preparation of pure oligonucleotide-alkaline phosphatase conjugates," Nucl. Acids Res. (1993)23:5532-5533.
Egholm, M., et al., "PNA hybridizes to complementary oligonucleotides obeying the Watson-Crick hydrogen-bonding rules," Nature (1993) 365:566-568.
Nielsen, P.E., et al., "Sequence-Selective Recognition of DNA by Strand Displacement with a Thymine-Substituted Polyamide," Science (1991) 25:1497-1500.
Englisch, V.U., et al., "Chemisch modifizierte Oligonucleotide als Sonden und Agentien," Angewandte Chemie (1991) 103:629-646.

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